S
Satoshi Wakita
Researcher at Kogakuin University
Publications - 19
Citations - 364
Satoshi Wakita is an academic researcher from Kogakuin University. The author has contributed to research in topics: Chitin & Chitinase. The author has an hindex of 10, co-authored 18 publications receiving 266 citations.
Papers
More filters
Journal ArticleDOI
Acidic mammalian chitinase is a proteases-resistant glycosidase in mouse digestive system
Misa Ohno,Masahiro Kimura,Haruko Miyazaki,Kazuaki Okawa,Riho Onuki,Chiyuki Nemoto,Eri Tabata,Satoshi Wakita,Akinori Kashimura,Masayoshi Sakaguchi,Yasusato Sugahara,Nobuyuki Nukina,Nobuyuki Nukina,Nobuyuki Nukina,Peter O. Bauer,Fumitaka Oyama +15 more
TL;DR: Evidence is provided that acidic mammalian chitinase (AMCase) can function as a protease-resistant major glycosidase under the conditions of stomach and intestine and degrade chit in substrates to produce (GlcNAc)2, a source of carbon, nitrogen and energy.
Journal ArticleDOI
Chitin digestibility is dependent on feeding behaviors, which determine acidic chitinase mRNA levels in mammalian and poultry stomachs.
Eri Tabata,Akinori Kashimura,Azusa Kikuchi,Hiromasa Masuda,Ryo Miyahara,Yusuke Hiruma,Satoshi Wakita,Misa Ohno,Masayoshi Sakaguchi,Yasusato Sugahara,Vaclav Matoska,Peter O. Bauer,Fumitaka Oyama +12 more
TL;DR: The results indicate that feeding behavior affects Chia expression levels as well as chitinolytic activity of the enzyme, and determines chitin digestibility in the particular animals.
Journal ArticleDOI
Gastric and intestinal proteases resistance of chicken acidic chitinase nominates chitin-containing organisms for alternative whole edible diets for poultry
Eri Tabata,Akinori Kashimura,Satoshi Wakita,Misa Ohno,Masayoshi Sakaguchi,Yasusato Sugahara,Yoshihiro Kino,Vaclav Matoska,Peter O. Bauer,Fumitaka Oyama +9 more
TL;DR: Functional similarity of chicken Chia with the mouse enzyme suggests that chitin-containing organisms can be used for alternative poultry diets not only as whole edible resources but also as enhancers of their nutritional value.
Journal ArticleDOI
A simple procedure for preparing chitin oligomers through acetone precipitation after hydrolysis in concentrated hydrochloric acid
Nao Kazami,Masayoshi Sakaguchi,Daisuke Mizutani,Tatsuhiko Masuda,Satoshi Wakita,Fumitaka Oyama,Masao Kawakita,Masao Kawakita,Yasusato Sugahara +8 more
TL;DR: The acetone precipitation method is highly useful for recovering chit in oligomers from the acid hydrolysate of chitin, and a model that explains the process of oligomer accumulation is proposed.
Journal ArticleDOI
Improved fluorescent labeling of chitin oligomers: Chitinolytic properties of acidic mammalian chitinase under somatic tissue pH conditions
Satoshi Wakita,Masahiro Kimura,Naoki Kato,Akinori Kashimura,Shunsuke Kobayashi,Naoto Kanayama,Misa Ohno,Shotaro Honda,Masayoshi Sakaguchi,Yasusato Sugahara,Peter O. Bauer,Fumitaka Oyama +11 more
TL;DR: An improved method for chitin oligosaccharides suppressing this side reaction by pre-acidification of the fluorophore-labeling reaction mixture is established and it is found that AMCase produced dimer of N-acetyl-d-glucosamine (GlcNAc) at strong acidic to neutral condition.