Showing papers by "Mateus Matiuzzi da Costa published in 2004"

Atividade antimicrobiana "in vitro" de extrato alcóolico de própolis

Abstract: Propolis is a natural resin collected and modified by honeybees Since ancient times it has been used as a chemotherapeutic agent The propolis antibacterial activity was evaluated through bacterial inoculation on BHI agar plates with 5% of alcoholic propolis extract (10(6) bacteria mL-1) One hundred and sixty one bacterial strains were evaluated, as Gram positive bacteria (Staphylococcus sp, Streptococcus sp, Nocardia asteroides and Rhodococcus equi) as Gram negative (Escherichia coli, Salmonella sp, Proteus mirabilis and Pseudomonas aeruginosa) The strain was considered sensible to the propolis extract when no bacterial growth was evident on the plate after 72 hours at 37oC The control test used agar plates with 5% of ethanol (M2) and 5% of saline solution Propolis extract demonstrated antibacterial activity on 677% of the tested strains; 926% of Gram-positive and 425% of Gram-negative strains presented sensitivity The propolis extract showed effective antibacterial activity against the majority of tested strains

Evaluation of PCR based on gene apxIVA associated with 16S rDNA sequencing for the identification of Actinobacillus pleuropneumoniae and related species.

Abstract: The pleuropneumonia caused by Actinobacillus pleuropneumoniae (App) is one the most important swine respiratory diseases. Biochemical and serological tests are widely applied for App diagnosis and characterization. However, in some isolates, conflicting results are found. The present work focus on the characterization of 29 isolates biochemically classified as A. pleuropneumoniae, collected from swine in herds with or without a clinical history of pleuropneumonia. Sixteen isolates were from healthy swine, initially classified as nonserotypable A. pleuropneumoniae; they displayed differences in the molecular characterization patterns of App (genes cpx and apxI, II, and III). Those bacteria that could not be serotyped were submitted to rDNA 16S sequencing. All 29 isolates were analyzed by PCR for the presence of the apxIVA gene. Thirteen isolates (45%) were confirmed to be A. pleuropneumoniae by PCR, nine being from diseased animals (31%) and four from healthy animals (14%) with conclusive serotyping. The rDNA 16S sequencing was used to classify the other 16 isolates in related species other than A. pleuropneumoniae, resulting in eleven A. minor, three A. porcinus, and two Pasteurella sp. Because of conflicting results between biochemical tests and rDNA 16S sequencing, the biochemical characterization was repeated, and the new results were in agreement with the rDNA 16S sequencing data. Biochemical characterization proved to be efficient for the majority of the A. pleuropneumoniae isolates. Nevertheless, conventional tests can render conflicting results, and other methodologies, such as amplification of A. pleuropneumoniae specific apxIVA gene and rDNA 16S sequencing, are very useful for improved classification. We also observed a great variety in rDNA 16S sequences from different A. minor isolates.