Mauro Castellarin

TL;DR: Overabundance of Fusobacterium sequences in tumor versus matched normal control tissue is verified by quantitative PCR analysis from a total of 99 subjects, and a positive association with lymph node metastasis is observed.

TL;DR: HLAminer, a computational method for identifying HLA alleles directly from shotgun sequence datasets that circumvents the additional time and cost of generating HLA-specific data and capitalizes on the increasing accessibility and affordability of massively parallel sequencing.

TL;DR: T-cell antigen receptor (TCR) variability enables the cellular immune system to discriminate between self and non-self as mentioned in this paper, and T-cell repertoire sequencing is still in its infancy, however, it is expected that maturation of the field will involve the introduction of improved, standardized tools for data handling, deposition and statistical analysis, as well as the emergence of new and equivalently large-scale technologies for Tcell functional analysis and antigen discovery.

TL;DR: The findings reveal the limitations of spontaneous tumor immunity in the setting of standard treatments and suggest a high degree of ignorance of tumor mutations that could potentially be reversed by immunotherapy.

TL;DR: This nascent field and TCR-seq methodology, which involves the use of next generation sequencing platforms to generate large numbers of short DNA sequences covering key regions of the TCR coding sequence, is introduced and discusses recent insights into healthy and diseased TCR repertoires.