Gamma-aminobutyric acid (GABA), a four-carbon non-protein amino acid, is found in most prokaryotic and eukaryotic organisms. Although, ample research into GABA has occurred in mammals as it is a major inhibitory neurotransmitter; in plants, a role for GABA has often been suggested as a metabolite that changes under stress rather than as a signal, as no receptor or motif for GABA binding was identified until recently and many aspects of its biological function (ranging from perception to function) remain to be answered. In this review, flexible properties of GABA in regulation of plant responses to various environmental biotic and abiotic stresses and its integration in plant growth and development either as a metabolite or a signaling molecule are discussed. We have elaborated on the role of GABA in stress adaptation (i.e., salinity, hypoxia/anoxia, drought, temperature, heavy metals, plant–insect interplay and ROS-related responses) and its contribution in non-stress-related biological pathways (i.e., involvement in plant–microbe interaction, contribution to the carbon and nitrogen metabolism and governing of signal transduction pathways). This review aims to represent the multifunctional contribution of GABA in various biological and physiological mechanisms under stress conditions; the objective is to review the current state of knowledge about GABA role beyond stress-related responses. Our effort is to place findings about GABA in an organized and broader context to highlight its shared metabolic and biologic functions in plants under variable conditions. This will provide potential modes of GABA crosstalk in dynamic plant cell responses.

Diverse role of γ-aminobutyric acid in dynamic plant cell responses.

In plants dehydration imposed by salinity can invoke physical changes at the interface of the plasma membrane and cell wall. Changes in hydrostatic pressure activate ion channels and cause depolarization of the plasma membrane due to disturbance in ion transport. During the initial phases of salinity stress, the relatively high osmotic potential of the rhizosphere enforces the plant to use a diverse spectrum of strategies to optimize water and nutrient uptake. Signals of salt stress are recognized by specific root receptors that activate an osmosensing network. Plant response to hyperosmotic tension is closely linked to the calcium (Ca2+) channels and interacting proteins such as calmodulin. A rapid rise in cytosolic Ca2+ levels occurs within seconds of exposure to salt stress. Plants employ multiple sensors and signaling components to sense and respond to salinity stress, of which most are closely related to Ca2+ sensing and signaling. Several tolerance strategies such as osmoprotectant accumulation, antioxidant boosting, polyaminses and nitric oxide (NO) machineries are also coordinated by Ca2+ signaling. Substantial research has been done to discover the salt stress pathway and tolerance mechanism in plants, resulting in new insights into the perception of salt stress and the downstream signaling that happens in response. Nevertheless, the role of multifunctional components such as Ca2+ has not been sufficiently addressed in the context of salt stress. In this review, we elaborate that the salt tolerance signaling pathway converges with Ca2+ signaling in diverse pathways. We summarize knowledge related to different dimensions of salt stress signaling pathways in the cell by emphasizing the administrative role of Ca2+ signaling on salt perception, signaling, gene expression, ion homeostasis and adaptive responses.

https://www.tandfonline.com/doi/pdf/10.1080/15592324.2019.1665455

Calcium signaling and salt tolerance are diversely entwined in plants.

Postharvest diseases significantly reduce the shelf-life of harvested fruits/vegetables worldwide. Bacillus spp. are considered to be an eco-friendly and bio-safe alternative to traditional chemical fungicides/bactericides due to their intrinsic ability to induce native anti-stress pathways in plants. This review compiles information from multiple scientific databases (Scopus, ScienceDirect, GoogleScholar, ResearchGate, etc.) using the keywords “postharvest diseases”, “Bacillus”, “Bacillus subtilis”, “biocontrol”, “storage”, “losses”, and “fruits/vegetables”. To date, numerous examples of successful Bacillus spp. application in controlling various postharvest-emerged pathogens of different fruits/vegetables during handling, transportation, and storage have been described in the literature. The mechanism/s of such action is/are still largely unknown; however, it is suggested that they include: i) competition for space/nutrients with pathogens; ii) production of various bio-active substances with antibiotic activity and cell wall-degrading compounds; and iii) induction of systemic resistance. With that, Bacillus efficiency may depend on various factors including strain characteristics (epiphytes or endophytes), application methods (before or after harvest/storage), type of pathogens/hosts, etc. Endophytic B. subtilis-based products can be more effective because they colonize internal plant tissues and are less dependent on external environmental factors while protecting cells inside. Nevertheless, the mechanism/s of Bacillus action on harvested fruits/vegetables is largely unknown and requires further detailed investigations to fully realize their potential in agricultural/food industries.

https://www.mdpi.com/2223-7747/8/4/97/pdf

Bacillus Spp.: Efficient Biotic Strategy to Control Postharvest Diseases of Fruits and Vegetables

Optimal control of environmental conditions affecting lettuce plant growth in a controlled environment with artificial lighting: A review

Salt stress is a serious abiotic stress that caused crop growth inhibition and yield decline. Previous studies have reported on the the synthesis of gamma-aminobutyric acid (GABA) and its relationship with plant resistance under various abiotic stress. However, the relationship between exogenous GABA alleviating plant salt stress damage and ion flux, amino acid synthesis, and key enzyme expression remains largely unclear. We investigated plant growth, Na+ transportation and accumulation, reactive oxygen species (ROS) metabolism and evaluated the effect of GABA on amino acids, especially SlGADs gene expression and the endogenous GABA content of tomato (Solanum lycopersicum L.) seedlings treated with or without 5 mmol·L− 1 GABA under 175 mmol·L− 1 NaCl stress. Exogenous application of GABA significantly reduced the salt damage index and increased plant height, chlorophyll content and the dry and fresh weights of tomato plants exposed to NaCl stress. GABA significantly reduced Na+ accumulation in leaves and roots by preventing Na+ influx in roots and transportation to leaves. The transcriptional expression of SlGAD1–3 genes were induced by NaCl stress especially with GABA application. Among them, SlGAD1 expression was the most sensitive and contributed the most to the increase in glutamate decarboxylase (GAD) activity induced by NaCl and GABA application; Exogenous GABA increased GAD activity and amino acid contents in tomato leaves compared with the levels under NaCl stress alone, especially the levels of endogenous GABA, proline, glutamate and eight other amino acids. These results indicated that SlGADs transcriptional expression played an important role in tomato plant resistance to NaCl stress with GABA application by enhancing GAD activity and amino acid contents. GABA significantly alleviated the active oxygen-related injury of leaves under NaCl stress by increasing the activities of antioxidant enzymes and decreasing the contents of active oxygen species and malondialdehyde. Exogenous GABA had a positive effect on the resistance of tomato seedlings to salt stress, which was closely associated with reducing Na+ flux from root to leaves, increasing amino acid content and strengthening antioxidant metabolism. Endogenous GABA content was induced by salt and exogenous GABA at both the transcriptional and metabolic levels.

/pdf/gamma-aminobutyric-acid-gaba-alleviates-salt-damage-in-1jkm1a3zhc.pdf

Gamma-aminobutyric acid (GABA) alleviates salt damage in tomato by modulating Na + uptake, the GAD gene, amino acid synthesis and reactive oxygen species metabolism

Photosynthesis is defined as a light-dependent process; however, it is negatively influenced by high light (HL) intensities. To investigate whether the memory of growth under monochromatic or combinational lights can influence plant responses to HL, rose plants were grown under different light spectra [including red (R), blue (B), 70:30 % red:blue (RB) and white (W)] and were exposed to HL (1500 μmol m-2 s-1) for 12 h. Polyphasic chlorophyll a fluorescence (OJIP) transients revealed that although monochromatic R- and B-grown plants performed well under control conditions, the functionality of their electron transport system was more sensitive to HL than that of the RB- and W-grown plants. Before exposure to HL, the highest anthocyanin concentration was observed in R- and B-grown plants, while exposure to HL reduced anthocyanin concentration in both R- and B-grown plants. Ascorbate peroxidase and catalase activities decreased, while superoxide dismutase activity was increased after exposure to HL. This caused an increase in H2O2 concentration and malondialdehyde content following HL exposure. Soluble carbohydrates were decreased by exposure to HL, and this decrease was more emphasized in R- and B-grown plants. In conclusion, growing plants under monochromatic light reduced the plants ability to cope with HL stress.

Effects of growth under different light spectra on the subsequent high light tolerance in rose plants

Title: Enhanced salt tolerance and photosynthetic performance: Implication of ɤ-amino butyric acid application in salt-exposed lettuce (Lactuca sativa L.) plants

Gamma-Aminobutyric acid (GABA) accumulates in plants following exposure to heavy metals. To investigate the role of GABA in cadmium (Cd) tolerance and elucidate the underlying mechanisms, GABA (0, 25 and 50 µM) was applied to Cd-treated maize plants. Vegetative growth parameters were improved in both Cd-treated and control plants due to GABA application. Cd uptake and translocation were considerably inhibited by GABA. Antioxidant enzyme activity was enhanced in plants subjected to Cd. Concurrently GABA caused further increases in catalase and superoxide dismutase activities, which led to a significant reduction in hydrogen peroxide, superoxide anion and malondealdehyde contents under stress conditions. Polyamine biosynthesis-responsive genes, namely ornithine decarboxylase and spermidine synthase, were induced by GABA in plants grown under Cd shock. GABA suppressed polyamine oxidase, a gene related to polyamine catabolism, when plants were exposed to Cd. Consequently, different forms of polyamines were elevated in Cd-exposed plants following GABA application. The maximum quantum efficiency of photosystem II (Fv/Fm) was decreased by Cd-exposed plants, but was completely restored by GABA to the same value in the control. These results suggest a multifaceted contribution of GABA, through regulation of Cd uptake, production of reactive oxygen species and polyamine metabolism, in response to Cd stress.

/pdf/g-aminobutyric-acid-confers-cadmium-tolerance-in-maize-5ec4683ory.pdf

γ-Aminobutyric acid confers cadmium tolerance in maize plants by concerted regulation of polyamine metabolism and antioxidant defense systems

Light emitting diodes (LEDs) now enable precise light quality control. Prior to commercialisation however, the plant response to the resultant light quality regime ought to be addressed. The response was examined here in chrysanthemum by evaluating growth, chlorophyll fluorescence (before and following water deficit), as well as stomatal anatomy (density, size, pore dimensions and aperture heterogeneity) and closing ability. Plants were grown under blue (B), red (R), a mixture of R (70%) and B (RB), or white (W; 41% B, 39% intermediate spectrum, 20% R) light LEDs. Although R light promoted growth, it also caused leaf deformation (epinasty) and disturbed the photosynthetic electron transport system. The largest stomatal size was noted following growth under B light, whereas the smallest under R light. The largest stomatal density was observed under W light. Monochromatic R light stimulated both the rate and the degree of stomatal closure in response to desiccation compared with the other light regimes. We conclude that stomatal size is mainly controlled by the B spectrum, whereas a broader spectral range is important for determining stomatal density. Monochromatic R light enhanced stomatal ability to regulate water loss upon desiccation.

Monochromatic red light during plant growth decreases the size and improves the functionality of stomata in chrysanthemum

Light spectrum of growing environment is a determinant factor for plant growth and photosynthesis. Plants under different light spectra exhibit different growth and photosynthetic behaviors. To unravel the effects of light spectra on plant growth, photosynthetic pigments and electron transport chain reactions, purple and green basil varieties were grown under five different light spectra including white (W: 400–730 nm), blue (B: 400–500 nm), red (R: 600–700 nm) and two combinations of R and B lights (R50B50 and R70B30), with same PPFD (photosynthetic photon flux density). Almost all values for shoot and root growth traits were higher in purple variety and were improved by combinational R and B lights (especially under R70B30), while they were negatively influenced by B monochromatic light when compared to growth traits of W-grown plants. Highest concentration of photosynthetic pigments was detected in R70B30. Biophysical properties of photosynthetic electron transport chain showed higher florescence intensity at all steps of OJIP kinetics in plants grown under R light in both varieties. Oxygen evolving complex activity (Fv/Fo) and PSII maximum quantum efficiency (Fv/Fm) in R-grown plants were lower than plants grown under other light spectra. Values for parameters related to specific energy fluxes per reaction center (ABS/RC, TRo/RC, ETo/RC and DIo/RC) were increased under R light (especially for purple variety). Performance index was significantly decreased under R light in both varieties. In conclusion, light spectra other than RB combination, induced various limitations on pigmentations, efficiency of electron transport and growth of basil plants and the responses were cultivar specific.

/pdf/photosynthetic-and-growth-responses-of-green-and-purple-4816ltvbkw.pdf

Mehdi Seif

Papers

Effects of growth under different light spectra on the subsequent high light tolerance in rose plants

Title: Enhanced salt tolerance and photosynthetic performance: Implication of ɤ-amino butyric acid application in salt-exposed lettuce (Lactuca sativa L.) plants

γ-Aminobutyric acid confers cadmium tolerance in maize plants by concerted regulation of polyamine metabolism and antioxidant defense systems

Monochromatic red light during plant growth decreases the size and improves the functionality of stomata in chrysanthemum

Photosynthetic and growth responses of green and purple basil plants under different spectral compositions