M
Melanie Hirz
Researcher at Graz University of Technology
Publications - 6
Citations - 853
Melanie Hirz is an academic researcher from Graz University of Technology. The author has contributed to research in topics: Pichia pastoris & Membrane protein. The author has an hindex of 4, co-authored 6 publications receiving 704 citations. Previous affiliations of Melanie Hirz include Institute of Molecular Biotechnology.
Papers
More filters
Journal ArticleDOI
Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production.
TL;DR: This review refers to established tools in protein expression in P. pastoris and highlights novel developments in the areas of expression vector design, host strain engineering and screening for high-level expression strains.
Journal ArticleDOI
A novel cholesterol-producing Pichia pastoris strain is an ideal host for functional expression of human Na,K-ATPase α3β1 isoform
TL;DR: Evidence is provided that the humanized sterol composition positively influenced Na,K-ATPase α3β1 stability, activity, and localization to the yeast plasma membrane, indicating that cholesterol-producing yeast will have high potential for functional expression of many mammalian membrane proteins.
Journal ArticleDOI
Overexpression of membrane proteins from higher eukaryotes in yeasts
TL;DR: An overview on the remarkable progress, and discusses pitfalls, in applying various yeast host strains for high-level expression of eukaryotic membrane proteins is provided.
Journal ArticleDOI
Perdeuteration of cholesterol for neutron scattering applications using recombinant Pichia pastoris.
Martine Moulin,Gernot Strohmeier,Melanie Hirz,Katherine C. Thompson,Adrian R. Rennie,Richard A. Campbell,Harald Pichler,Selma Maric,V. Trevor Forsyth,Michael Haertlein +9 more
TL;DR: A procedure for in vivo production of perdeuterated recombinant cholesterol in lipid-engineered Pichia pastoris using flask and fed-batch fermenter cultures in deuterated minimal medium is described.
Book ChapterDOI
Membrane protein production in yeast: Modification of yeast membranes for human membrane protein production
Anita Emmerstorfer-Augustin,Tamara Wriessnegger,Melanie Hirz,Guenther Zellnig,Harald Pichler +4 more
TL;DR: This chapter describes how to engineer the yeast Pichia pastoris to obtain humanized sterol compositions by implementing some simple genetic engineering approaches, and shows how P. pastoris can be reprogrammed to mainly produce cholesterol instead of ergosterol.