Melissa S. Tassinari

TL;DR: The relationship of cell proliferation to the temporal expression of genes characterizing a developmental sequence associated with bone cell differentiation was examined in primary diploid cultures of fetal calvarial derived osteoblasts by the combined use of autoradiography, histochemistry, biochemistry, and mRNA assays of osteoblast cell growth and phenotypic genes.

TL;DR: In this article, the relationship of cell proliferation to the temporal expression of genes characterizing a developmental sequence associated with bone cell differentiation was examined in primary diploid cultures of fetal calvarial derived osteoblasts by the combined use of autoradiography, histochemistry, biochemistry, and mRNA assays of osteoblast cell growth and phenotypic genes.

TL;DR: The present studies suggest that formation of a collagenous matrix, dependent on ascorbic acid, is requisite for expression of the osteoblast phenotype, as reflected by a 200‐fold increase in osteocalcin synthesis.

TL;DR: It is proposed that the glucocorticoid promote changes in gene expression involved in cell‐cell and cell‐extracellular matrix signaling mechanism that support the growth and differentiation of cells capable of osteoblast phenotype development and bone tissue‐like organization, while inhibiting the growth of cells that cannot progress to the mature osteoblow phenotype in fetal rat calvarial cultures.

TL;DR: The purpose of this review is to identify the critical events in human postnatal bone growth and to compare human bone development to that of other species.