The influence of diet on the distribution of nitrogen isotopes in animals was investigated by analyzing animals grown in the laboratory on diets of constant nitrogen isotopic composition. 
The isotopic composition of the nitrogen in an animal reflects the nitrogen isotopic composition of its diet. The δ^(15)N values of the whole bodies of animals are usually more positive than those of their diets. Different individuals of a species raised on the same diet can have significantly different δ^(15)N values. The variability of the relationship between the δ^(15)N values of animals and their diets is greater for different species raised on the same diet than for the same species raised on different diets. Different tissues of mice are also enriched in ^(15)N relative to the diet, with the difference between the δ^(15)N values of a tissue and the diet depending on both the kind of tissue and the diet involved. The δ^(15)N values of collagen and chitin, biochemical components that are often preserved in fossil animal remains, are also related to the δ^(15)N value of the diet. 
The dependence of the δ^(15)N values of whole animals and their tissues and biochemical components on the δ^(15)N value of diet indicates that the isotopic composition of animal nitrogen can be used to obtain information about an animal's diet if its potential food sources had different δ^(15)N values. The nitrogen isotopic method of dietary analysis probably can be used to estimate the relative use of legumes vs non-legumes or of aquatic vs terrestrial organisms as food sources for extant and fossil animals. However, the method probably will not be applicable in those modern ecosystems in which the use of chemical fertilizers has influenced the distribution of nitrogen isotopes in food sources. 
The isotopic method of dietary analysis was used to reconstruct changes in the diet of the human population that occupied the Tehuacan Valley of Mexico over a 7000 yr span. Variations in the δ^(15)C and δ^(15)N values of bone collagen suggest that C_4 and/or CAM plants (presumably mostly corn) and legumes (presumably mostly beans) were introduced into the diet much earlier than suggested by conventional archaeological analysis.

Influence of Diet On the Distribtion of Nitrogen Isotopes in Animals

Positive ions that are available in soils absorb on grain surfaces. The total sum of cations that can be absorbed bij a soil/sediment at a certain PH is defined by the cation-exchange capacity (CEC, in meq g-1: mol equivalents per gram). The uptake of cations is an important parameter in agriculture and the larger the CEC, the more cations can be absorbed to the soil. The CEC depends highly on the pH of soil and sediments, where the CEC decreases with decreasing PH (increasing acidity). The exchange of ions on sediments occurs commonly fast on geological time scales, but the kinetics of adsorption in natural environments is still poorly understood. The strength of the bonding between the cations and the sediments varies from weak Van der Waals bondings (physical adsorption) to strong chemical bonds. The CEC is widely used for agricultural assessment because it is a measure of general soil fertility as well as an indicator of structural stability because CED is capabel of enhancing development of shrinkage cracks. The list below shows the CEC for different types of minerals. The data indicate that the CEC can vary over 2 orders of magnitude for various types of , minerals and can vary one order of magnitude within one soil type. Cation exchange capacity for different types of sediment (Eisma, 1992; Locher and de Bakker, 1990):

Cation exchange capacity.

The rapid determination of algal chlorophyll and carotenoid pigments and their breakdown products in natural waters by reverse-phase high-performance liquid chromatography

The microphytobenthos consists of unicellular eukaryotic algae and cyanobacteria that grow within the upper several millimeters of illuminated sediments, typically appearing only as a subtle brownish or greenish shading. The surficial layer of the sediment is a zone of intense microbial and geochemical activity and of considerable physical reworking. In many shallow ecosystems, the biomass of benthic microalgae often exceeds that of the phytoplankton in the overlying waters. Direct comparison of the abundance of benthic and suspended microalgae is complicated by the means used to measure biomass and by the vertical and horizontal distribution of the microphytobenthos in the sediment. Where biomass has been estimated as chlorophyll a, there may be negligible to large (40%) error due to interference by degradation products, except where chlorophyll is measured by high-performance liquid chromatography. The vertical distribution of microphytobenthos, aside from mat-forming species, is determined by the opposing effects of their vertical migration, which tends to concentrate them near the surface, and physical mixing by overlying currents, which tends to cause an even vertical distribution through the mixed layer of sediment. Uncertainties in vertical distribution are compounded by frequently patchy horizontal distribution. Under-sampling on small (<1 m) scales can lead to errors in the estimate that are comparable to the ranges of seasonal and geographic variation. These uncertainties are compounded by biases in the techniques used to estimate production by the microphytobenthos. In most environments studied, biomass (as chlorophyll a) and light availability appear to be the principal determinants of benthic primary production. The effect of variable light intensities on integral production can be described by a functional response curve. When normalized to the chlorophyll content of the surficial sediment, the residual variation in the data described by the functional response curve is due to changes in the chlorophyll-specific response to irradiance. Production by the benthos is often a significant fraction of production in the water column and microphytobenthos may contribute directly to water column production when they are resuspended. Thus on both the basis of biomass and biogeochemical reactivity, benthic microalgae play significant roles in system productivity and trophic dynamics, as well as such habitat characteristics as sediment stability. *** DIRECT SUPPORT *** A01BY074 00003

https://www.jstor.org/stable/info/1352224

Microphytobenthos: The ecological role of the “secret garden” of unvegetated, shallow-water marine habitats. I. Distribution, abundance and primary production

A review of the occurrence, analyses, toxicity, and biodegradation of naphthenic acids.

Characterization of naphthenic acids in oil sands wastewaters by gas chromatography-mass spectrometry.

Acute and subchronic mammalian toxicity of naphthenic acids from oil sands tailings

Estimating the in situ biodegradation of naphthenic acids in oil sands process waters by HPLC/HRMS

Naphthenic acids (NAs) are natural constituents in many petroleum sources, including bitumen in the oil sands of Northern Alberta, Canada. Bitumen extraction processes produce tailings waters that cannot be discharged to the environment because NAs are acutely toxic to aquatic species. However, aerobic biodegradation reduces the toxic character of NAs. In this study, four commercial NAs and the NAs in two oil sands tailings waters were characterized by gas chromatography−mass spectrometry. These NAs were also incubated with microorganisms in the tailings waters under aerobic, laboratory conditions. The NAs in the commercial preparations had lower molecular masses than the NAs in the tailings waters. The commercial NAs were biodegraded within 14 days, but only about 25% of the NAs native to the tailings waters were removed after 40−49 days. These results show that low molecular mass NAs (C ≤ 17) are more readily biodegraded than high molecular mass NAs (C ≥ 18). Moreover, the results indicate that biodegra...

Naphthenic acids in athabasca oil sands tailings waters are less biodegradable than commercial naphthenic acids.

Organic acids, similar in structure to naphthenic acids, have been associated with the acute toxicity of tailings produced by the oil sands industry in northeastern Alberta, Canada. Bacterial cultu...

Michael D. MacKinnon

Papers

Characterization of naphthenic acids in oil sands wastewaters by gas chromatography-mass spectrometry.

Acute and subchronic mammalian toxicity of naphthenic acids from oil sands tailings

Estimating the in situ biodegradation of naphthenic acids in oil sands process waters by HPLC/HRMS

Naphthenic acids in athabasca oil sands tailings waters are less biodegradable than commercial naphthenic acids.

Biodegradation of naphthenic acids by microbial populations indigenous to oil sands tailings