Detection of activated platelets in whole blood using activation- dependent monoclonal antibodies and flow cytometry

The general concept of using the unique phase separation behavior of some surfactant micelle solutions as a means for extractive separation is outlined and described. Next, the specific micellar parameters and phase separation behavior of nonionic and zwitterionic charge-type surfactant solutions are summarized. In addition, the phase behavior of some derivatized B-cyclodextrin solutions is briefly described. The specific applications of such systems and their phase behavior for the extractive preconcentration, separation, and/or purification of metal chelates, biomaterials, and organic compounds are summarized and discussed. The potential use of affinity ligands in conjunction with the cloud-point approach for selective bioextractions is also mentioned. The experimental protocols, advantages, and limitations of the different cloud-point extraction techniques are outlined. The use of zwitterionic as opposed to nonionic surfactant media in such separations is compared and contrasted. In addition, ...

A Critical Review of Surfactant-Mediated Phase Separations (Cloud-Point Extractions): Theory and Applications

The structural and functional properties of thrombospondin

The inherited platelet disorders are an uncommon cause of symptomatic bleeding. They may be difficult to diagnose (and are likely to be under-diagnosed) and pose problems in management. This review discusses the inherited platelet disorders summarising the current state of the art with respect to investigation and diagnosis and suggests how to manage bleeding manifestations with particular attention to surgical interventions and the management of pregnancy.

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A review of inherited platelet disorders with guidelines for their management on behalf of the UKHCDO

Michael E. Dockter

Papers

Hydrogenation of Triton X-100 eliminates its fluorescence and ultraviolet light absorption while preserving its detergent properties

Analysis of human platelet glycoproteins IIb-IIIa and Glanzmann's thrombasthenia in whole blood by flow cytometry

An improved method for determining the actin filament content of nonmuscle cells by the DNase I inhibition assay

The Wistar Furth rat: an animal model of hereditary macrothrombocytopenia.

Secreted platelet thrombospondin binds monovalently to platelets and erythrocytes in the absence of free Ca2