Michael K. Dush

TL;DR: The efficacy of this cDNA cloning strategy was demonstrated by isolating cDNA clones of mRNA from int-2, a mouse gene that expresses four different transcripts at low abundance, the longest of which is approximately 2.9 kilobases.

TL;DR: The nucleotide sequence of a functional mouse adenine phosphoribosyltransferase (APRT) gene and its cDNA is determined and the amino acid sequence of the enzyme is deduced from an open reading frame in the cDNA and predicts a protein with a molecular weight of 19,560.

TL;DR: Comparison between human and mouse APRT gene nucleotide sequences reveals a high degree of homology within protein coding regions but an absence of significant homology in 5' flanking, 3' untranslated, and intron sequences, suggesting that there may be selection for CpG dinucleotides in these regions and that their maintenance may be important for AP RT gene function.

TL;DR: A complete human APRT gene has been isolated from a lambda phage genomic library using cloned mouse APRT DNA as a probe and no gross deletions or rearrangements were revealed, nor the Taq1 polymorphism.

TL;DR: No upstream anti-sense transcripts were detected in either mouse CAK or liver cells, confirming that the mouse aprt promoter, unlike some other GC-rich promoters appears not to support bidirectional transcription.