Michaela Amon

TL;DR: Future directions of research should focus on the creation of microvascular networks within 3D tissue constructs in vitro before implantation or by co-stimulation of angiogenesis and parenchymal cell proliferation to engineer the vascularized tissue substitute in situ.

TL;DR: Experimental studies have now shown that efficient protection from ischaemic necrosis can also be achieved by remote preconditioning or pretreatment with chemical agents and growth factors, which mimic the action of surgical delay and ischaemia preconditionsing.

TL;DR: This new model enables repetitive and simultaneous in vivo microscopic evaluation of microvascular hypoperfusion, apoptosis, and tissue necrosis in a random pattern flap and represents an ideal tool to study novel protective strategies, including induction of angiogenesis, heat shock proteins, and HIF-1alpha.

TL;DR: Evidence is provided that metabolites of the HO and the NOS pathway mediate the cooling‐associated protection of inflamed tissue, whereas a coordinated function of the gaseous monoxides prevents microcirculatory dysfunction and apoptotic cell death.

TL;DR: Local heat preconditioning of myocutaneous tissue markedly increases flap survival by maintaining adequate nutritive perfusion rather than inducing ischemic tolerance, mediated through the carbon monoxide-associated vasoactive properties of HSP-32.