Showing papers by "Michaela Vorlíčková published in 2016"
TL;DR: The p53 tumour suppressor protein binds specifically to the G-quadruplex DNA formed by MYC promoter sequence, and it is proposed that p53 binding to G- quadruplexes can participate in regulation of p53 target genes.
Abstract: G-quadruplexes are four-stranded nucleic acid structures that are implicated in the regulation of transcription, translation and replication. Genome regions enriched in putative G-quadruplex motifs include telomeres and gene promoters. Tumour suppressor p53 plays a critical role in regulatory pathways leading to cell cycle arrest, DNA repair and apoptosis. In addition to transcriptional regulation mediated via sequence-specific DNA binding, p53 can selectively bind various non-B DNA structures. In the present study, wild-type p53 (wtp53) binding to G-quadruplex formed by MYC promoter nuclease hypersensitive element (NHE) III1 region was investigated. Wtp53 binding to MYC G-quadruplex is comparable to interaction with specific p53 consensus sequence (p53CON). Apart from the full-length wtp53, its isolated C-terminal region (aa 320-393) as well, is capable of high-affinity MYC G-quadruplex binding, suggesting its critical role in this type of interaction. Moreover, wtp53 binds to MYC promoter region containing putative G-quadruplex motif in two wtp53-expressing cell lines. The results suggest that wtp53 binding to G-quadruplexes can take part in transcriptional regulation of its target genes.
29 citations
TL;DR: This is the first study on the interaction of wild-type p53 with guanine quadruplexes formed by the human telomere sequence and it is shown that p53 binding to telomeric G-quadruplexes increases with the number of telomersic repeats.
13 citations