Nitrogen is the most limiting nutrient for crop production in many of the world's agricultural areas and its efficient use is important for the economic sustainability of cropping systems Furthermore, the dynamic nature of N and its propensity for loss from soil‐plant systems creates a unique and challenging environment for its efficient management Crop response to applied N and use efficiency are important criteria for evaluating crop N requirements for maximum economic yield Recovery of N in crop plants is usually less than 50% worldwide Low recovery of N in annual crop is associated with its loss by volatilization, leaching, surface runoff, denitrification, and plant canopy Low recovery of N is not only responsible for higher cost of crop production, but also for environmental pollution Hence, improving N use efficiency (NUE) is desirable to improve crop yields, reducing cost of production, and maintaining environmental quality To improve N efficiency in agriculture, integrated N management strategies that take into consideration improved fertilizer along with soil and crop management practices are necessary Including livestock production with cropping offers one of the best opportunities to improve NUE Synchrony of N supply with crop demand is essential in order to ensure adequate quantity of uptake and utilization and optimum yield This paper discusses N dynamics in soil‐plant systems, and outlines management options for enhancing N use by annual crops

Enhancing nitrogen use efficiency in crop plants

Fungi are major ecological players in both terrestrial and aquatic environments by cycling organic matter and channelling nutrients across trophic levels. High-throughput sequencing (HTS) studies of fungal communities are redrawing the map of the fungal kingdom by hinting at its enormous - and largely uncharted - taxonomic and functional diversity. However, HTS approaches come with a range of pitfalls and potential biases, cautioning against unwary application and interpretation of HTS technologies and results. In this Review, we provide an overview and practical recommendations for aspects of HTS studies ranging from sampling and laboratory practices to data processing and analysis. We also discuss upcoming trends and techniques in the field and summarize recent and noteworthy results from HTS studies targeting fungal communities and guilds. Our Review highlights the need for reproducibility and public data availability in the study of fungal communities. If the associated challenges and conceptual barriers are overcome, HTS offers immense possibilities in mycology and elsewhere.

Mycobiome diversity: high-throughput sequencing and identification of fungi.

Factors affecting the postharvest soluble solids and sugar content of tomato (Solanum lycopersicum L.) fruit

Magnesium (Mg) deficiency exerts a major influence on the partitioning of dry matter and carbohydrates between shoots and roots. One of the very early reactions of plants to Mg deficiency stress is the marked increase in the shoot-to-root dry weight ratio, which is associated with a massive accumulation of carbohydrates in source leaves, especially of sucrose and starch. These higher concentrations of carbohydrates in Mg-deficient leaves together with the accompanying increase in shoot-to-root dry weight ratio are indicative of a severe impairment in phloem export of photoassimilates from source leaves. Studies with common bean and sugar beet plants have shown that Mg plays a fundamental role in phloem loading of sucrose. At a very early stage of Mg deficiency, phloem export of sucrose is severely impaired, an effect that occurs before any noticeable changes in shoot growth, Chl concentration or photosynthetic activity. These findings suggest that accumulation of carbohydrates in Mg-deficient leaves is caused directly by Mg deficiency stress and not as a consequence of reduced sink activity. The role of Mg in the phloem-loading process seems to be specific; resupplying Mg for 12 or 24 h to Mg-deficient plants resulted in a very rapid recovery of sucrose export. It appears that the massive accumulation of carbohydrates and related impairment in photosynthetic CO2 fixation in Mg-deficient leaves cause an over-reduction in the photosynthetic electron transport chain that potentiates the generation of highly reactive O2 species (ROS). Plants respond to Mg deficiency stress by marked increases in antioxidative capacity of leaves, especially under high light intensity, suggesting that ROS generation is stimulated by Mg deficiency in chloroplasts. Accordingly, it has been found that Mg-deficient plants are very susceptible to high light intensity. Exposure of Mg-deficient plants to high light intensity rapidly induced leaf chlorosis and necrosis, an outcome that was effectively delayed by partial shading of the leaf blade, although the Mg concentrations in different parts of the leaf blade were unaffected by shading. The results indicate that photooxidative damage contributes to development of leaf chlorosis under Mg deficiency, suggesting that plants under high-light conditions have a higher physiological requirement for Mg. Maintenance of a high Mg nutritional status of plants is, thus, essential in the avoidance of ROS generation, which occurs at the expense of inhibited phloem export of sugars and impairment of CO2 fixation, particularly under high-light conditions.

/pdf/role-of-magnesium-in-carbon-partitioning-and-alleviating-87j5hsqlqi.pdf

Role of magnesium in carbon partitioning and alleviating photooxidative damage

Evaluation methods for assessing effectiveness of in situ remediation of soil and sediment contaminated with organic pollutants and heavy metals.

Soil pollution with heavy metals is a worldwide environmental problem. Phytoremediation through phytoextraction and phytostabilization appears to be a promising technology for the remediation of polluted soils. It is important to strongly emphasize that the ultimate goal of a heavy metal remediation process must be not only to remove the heavy metals from the soil (or instead to reduce their bioavailability and mobility) but also to restore soil quality. Soil quality is defined as the capacity of a given soil to perform its functions. Soil microbial properties are increasingly being used as biological indicators of soil quality due to their quick response, high sensitivity, and, above all, capacity to provide information that integrates many environmental factors. Indeed, microbial properties are among the most ecologically relevant indicators of soil quality. Consequently, microbial monitoring of the recovery of soil quality is often carried out during heavy metal phytoremediation processes. However, soil microbial properties are highly context dependent and difficult to interpret. For a better interpretation of microbial properties as indicators of soil quality, they may be grouped within categories of higher ecological relevance, such as soil functions, ecosystem health attributes, and ecosystem services.

Microbial Monitoring of the Recovery of Soil Quality During Heavy Metal Phytoremediation

The effects of variety and season on several organoleptic and nutritional quality parameters (i.e., dry weight (dw), total sugars, soluble solid compounds, titratable acidity (TA), electrical conductivity (EC), juiciness, firmness, vitamin C (vit C), total phenolic compounds, hydrophilic antioxidant capacity and minerals) of five different varieties of tomatoes (i.e., Jack, Cabrales, Jaguar, Iker and Nevada) grown in two crop cycles (spring and autumn) were studied. Each variety presented its own specific characteristics regarding the chosen parameters. Firmness, TA and EC were season dependent, whereas soluble solids content did not change between cycles. In some varieties, the dw, juiciness and total sugars were affected by climatic conditions. The total phenolic compounds and the hydrophilic antioxidant capacity were variety dependent in both cycles. By contrast, the vit C content was variety dependent only in the autumn cycle. Similarly, these latter parameters (phenolic compounds, hydrophilic antioxidant capacity and vit C) were also season dependent, showing higher values in the spring than in the autumn cycle. The effect of tomato variety and season on mineral contents is also discussed. Those tomatoes grown in the spring cycle had better quality according to the organoleptic parameters studied here as well as to a higher antioxidant capacity. The percentages of the recommended dietary allowances supplied by the studied tomatoes were not significantly affected by variety or season, despite differences in their physicochemical compositions.

https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1745-4557.2006.00053.x

Effects of variety and growth season on the organoleptic and nutritional quality of hydroponically grown tomato

BACKGROUND: A greenhouse experiment was performed to study the effect of cumulative air temperature and photosynthetically active radiation (PAR) on tomato quality. Tomato plants were subjected to two different shading treatments causing a 30 and 50% reduction in incoming PAR respectively (plants were exposed to 70 and 50% of incoming PAR respectively). Control plants (exposed to 100% of incoming PAR) were also included in the experiment. The experiment was carried out under unheated greenhouse conditions. To minimise the dependence of temperature on PAR, only a small area inside a large greenhouse was shaded, thereby allowing air currents to homogenise temperature all over the enclosure. Parameters of tomato quality were correlated with cumulative temperature (Tcum) and cumulative PAR (PARcum) for a period of 45 days before harvest. RESULTS:Tcum was strongly correlated with firmness, electrical conductivity, soluble solids content and total phenolic compounds and weakly correlated with pH, dry weight, titratable acidity and vitamin C content. PARcum was only weakly correlated with firmness, dry weight, soluble solids content and total phenolic compounds. CONCLUSION:Tcum has a stronger influence on tomato quality than PARcum. Growers could obtain tomatoes of similar quality under lower PAR than that provided by natural sunlight. Copyright © 2007 Society of Chemical Industry

Tomato quality is more dependent on temperature than on photosynthetically active radiation

The impact of nanoscale zero-valent iron particles on soil microbial communities is soil dependent

Summary The effect of magnesium (Mg 2+ )-deficiency on the antioxidant responses of Capsicum annuum was investigated over a 60-day period under controlled conditions. This Mg 2+ -deficiency aimed to mimic the physiological conditions that plants may experience in the field. At each harvest time, five different leaf-levels (L2 to L6) were distinguished. L2 and L6 correspond to the second and sixth youngest leaves, respectively. The following parameters were determined: Mg 2+ , chlorophyll and protein contents, total and redox pools of ascorbate and glutathione, and the activities of superoxide dismutase, ascorbate peroxidase, dehydroascorbate reductase, and glutathione reductase. Under Mg 2+ -deficiency, leaf Mg 2+ contents decreased over time in all leaf-levels except in the second youngest leaves (L2), where they remained constant at about 0.25% (dry weight basis). Mg 2+ -deficiency led to an increase in the antioxidant enzyme activities concomitant with an increase in the ascorbate and glutathione pools, whereas total chlorophyll and soluble protein contents decreased. The L2 leaves showed an increase in glutathione reductase activity and in the ascorbate redox state whereas no difference was observed for the other parameters. Superoxide dismutase activities increased in L5 leaves from day 15 and, afterwards, in L3 to L5 leaves, irrespective of Mg 2+ content. At day 30, glutathione reductase activities increased in L2 to L4 leaves and dehydroascorbate reductase activities in L4 leaves. At day 45, we observed an increase in the ascorbate peroxidase activities in L3 to L5 leaves. At the same time, ascorbate and glutathione pools increased in intermediate leaves, whereas chlorophyll content decreased in L3 and L4 leaves, and protein content decreased in L4 leaves. Results suggest that pepper leaves enhance their defence capacities against oxidative stress by increasing ascorbate more than glutathione synthesis. However, cells showed higher regeneration rates for the glutathione redox state than for the ascorbate redox state.

Mikel Anza

Papers

Microbial Monitoring of the Recovery of Soil Quality During Heavy Metal Phytoremediation

Effects of variety and growth season on the organoleptic and nutritional quality of hydroponically grown tomato

Tomato quality is more dependent on temperature than on photosynthetically active radiation

The impact of nanoscale zero-valent iron particles on soil microbial communities is soil dependent

Time course of antioxidant responses of Capsicum annuum subjected to a progressive magnesium deficiency