Mimi Healy

TL;DR: The modifications for automation included changes in rep-PCR chemistry and thermal cycling parameters, incorporation of microfluidics-based DNA amplicon fractionation and detection, and Internet-based computer-assisted analysis, reporting, and data storage.

TL;DR: The performance characteristics of the 5′NC methods were similar and both methods produced accurate results at the genotype level but neither method should be used for subtyping.

TL;DR: Automated rep-PCR may be a time-efficient, effective, easy-to-use, novel genotyping tool for identifying and determining the strain relatedness of fungi and may be useful for epidemiological studies, molecular typing, and surveillance of Aspergillus species.

TL;DR: DL is useful for screening MRSA isolates to rule out potential outbreaks of MRSA in hospitals, but PFGE provides better discrimination of potential outbreak strains and is more useful for confirming strain relatedness and specific USA types.

TL;DR: Rep-PCR dendrogram cluster analysis showed agreement with species sequence identification (elongation factor 1 alpha gene) in fusariosis isolates tested by the commercially available DiversiLab System.