Showing papers by "Mitsuo Miyazawa published in 1998"
TL;DR: There is little difference in metabolic pathway between the (+)- and (-)-forms of common cutworm larvae, according to spectroscopically analyzed metabolites.
Abstract: (+)-Limonene was mixed in an artificial diet at a concentration of 1 mg/g of diet, and the diet was fed to the last instar larvae of common cutworm (Spodoptera litura). Metabolites were recovered f...
35 citations
TL;DR: A methanol extract from Scrophulia ningpoensis showed suppressive effect of the SOS-inducing activity of the mutagen 2-(2-furyl)-3-(5-nitro-2)-acrylamide (furylfuramide) in the Salmonella typhimurium TA1535/pSK1002 umu test as mentioned in this paper.
Abstract: A methanol extract from Scrophulia ningpoensis showed suppressive effect of the SOS-inducing activity of the mutagen 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (furylfuramide) in the Salmonella typhimurium TA1535/pSK1002 umu test. The methanol extract was re-extracted with hexane, dichloromethane, butanol, and water. An acidic fraction of the dichloromethane fraction showed a suppressive effect. Suppressive compounds in the acidic fraction were isolated by SiO2 column chromatography and identified as trans-cinnamic acid (1), p-methoxycinnamic acid (2), 3,4-dimethoxycinnamic acid (3), and 4-hydroxy-3-methoxycinnamic acid (4) by GC, GC/MS, and 1H NMR spectroscopy. Compounds 1−4 suppressed the SOS-inducing activity of furylfuramide in the umu test. Compounds 1−4 suppressed 44, 31, 37, and 36% of the SOS-inducing activity at a concentration of 1.4 μmol/mL. These compounds were assayed with other mutagens, 4-nitroquinoline 1-oxide (4NQO) and N-methyl-N‘-nitro-N-nitrosoguanidine (MNNG). In addition, compounds 1−...
27 citations
TL;DR: In this article, inhibition of acetylcholinesterase (AChE) activity by 5 kinds of volatile α, β-unsaturated ketones was studied.
Abstract: Inhibition of acetylcholinesterase (AChE) activity by 5 kinds of volatile α, β-unsaturated ketones was studied. Inhibition of AChE was measured by the colorimetric method. α-Ionone showed the strongest inhibition.
12 citations
TL;DR: Insecticidal lignans gomisin B and Gomisin N were isolated from the n-hexane extract of fruits of Schisandra chinensis under bioassay guide of Drosophila melanogaster Meigen and showed the lethal dose for 50% mortality.
Abstract: Insecticidal lignans gomisin B and gomisin N were isolated from the n-hexane extract of fruits of Schisandra chinensis under bioassay guide of Drosophila melanogaster Meigen. Gomisin B and N showed the lethal dose for 50% mortality(LC50) at concentration of 0.031μmol/ml and 0.125μmol/ml, respectrively.
10 citations
TL;DR: In this article, the microbial transformation of (±)-lavandulol has been investigated using the plant pathogenic fungus Glomerella cingulata, and the structures of biotransformation products and metabolic pathways were clarified.
Abstract: To clarify the structures of biotransformation products and the metabolic pathways, the microbial transformation of (±)-lavandulol has been investigated using the plant pathogenic fungus Glomerella cingulata. (±)-Lavandulol [(±)-5-methyl-2-(1-methylethenyl)-4-hexen-1-ol] was mainly oxidized at the C-4 double bond to give (−)-(2S,4S)-1,5-epoxy-5-methyl-2-(1-methylethenyl)-4-hexanol and cis- and trans-1,4-epoxy-5-methyl-2-(1-methylethenyl)-5-hexanol. 5-Methyl-2-(1-methylethenyl)-4-hexene-1,6-diol (6-hydroxylavandulol) was also produced through this biotransformation. These structures were confirmed by the mass, IR, 1H NMR, and 13C NMR spectral data. Keywords: Biotransformation; microbial transformation; Glomerella cingulata; plant pathogenic fungus; (±)-lavandulol; tetrahydropyran; tetrahydrofuran
10 citations
TL;DR: The biotransformation of (+)-bicyclo by Aspergillus niger and Glomerella cingulata showed high optical purity and reduction by A. niger yielded optically active ( - )-(lR, 2S, 5R, 6S)-bicycleclo[3.3.I]nanane-2,6-diol respectively.
Abstract: The biotransformation of (+)-bicyclo[3.3.I]nonane-2,6-dione by Aspergillus niger and Glomerella cingulata was investigated. The diketone was reduced to the ketoalcohol 2-endo-hydroxy-bicyclo[3.3.1]nonane-6-one and the diol endo,endo-bicycla[3.3.I]nanane-2,6-diol respectively. Endo,endo-bicyclo[3.3.1]nonane-2,6-diol and ketoalcohols produced by G. cingulata had high optical purity, on the other hand, reduction by A. niger yielded optically active ( - )-(lR, 2S, 5R, 6S)-bicyclo[3.3.1]nonane-2,6-dial(99.9% e.e.).
7 citations
TL;DR: The microbial transformation of (2 Z ,6 Z )-farnesol was investigated using the plant pathogenic fungus, Glomerella cingulata as a biocatalyst to obtain two major metabolites and one further degraded compounds.
7 citations
TL;DR: The methanol extract from Machilus thunbergii showed a suppressive effect on umu gene expression of the SOS response in Salmonella typhimurium TA1535/pSK1002 against the mutagen, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1), which requires liver metabolizing enzymes.
Abstract: The methanol extract from Machilus thunbergii showed a suppressive effect on umu gene expression of the SOS response in Salmonella typhimurium TA1535/pSK1002 against the mutagen, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1), which requires liver metabolizing enzymes. The methanol extract from M. thunbergii was successively re-extracted with chloroform, butanol and water. A suppressive compound in the chloroform extract fraction was isolated by SiO2 column chromatography and identified as meso-dihydroguaiaretic acid by GC-MS, and 1H- and 13C-NMR spectroscopy. Meso-dihydroguaiaretic acid inhibited of the SOS-inducing activity of Trp-P-1 in the umu test. Gene expression was suppressed by 62% at less than 0.18 mumol/ml, the ID50 value being 0.08 mumol/ml. Compound 1 was also assayed with aflatoxin B1 (AfB1) and showed a suppressive effect.
6 citations