Showing papers by "Mitsuo Miyazawa published in 2007"
TL;DR: A tyrosinase inhibitor was isolated from the sprout of Polygonum hydropiper L. (Benitade) by activity-guided fractionation and compound 1 was more inhibited than the former and showed inhibitory effect equal to that of the latter.
Abstract: A tyrosinase inhibitor was isolated from the sprout of Polygonum hydropiper L. (Benitade) by activity-guided fractionation and identified as (2R,3R)-+-taxifolin (1) by spectroscopic means. Compound 1 inhibited 70% of tyrosinase activity at a concentration of 0.50 mM. ID50 (50% inhibition dose) value of compound 1 was 0.24 mM. As compared with tyrosinase inhibitor known cosmetic agent such as arbutin and kojic acid, compound 1 was more inhibited than the former and showed inhibitory effect equal to that of the latter. To study the inhibitory effect of (2R,3R)-+-taxifolin derivatives against tyrosinase activity, 3,7,3',4'-taxifolin tetraacetate (2) and 5,7,3',4'-taxifolin teramethyl ether (3) were also assayed together with compound 1.
58 citations
TL;DR: The components of the essential oil from sprouts of Polygonum hydropiper L. (Benitade) were analysed by capillary GC and GC-MS as discussed by the authors.
Abstract: The components of the essential oil from sprouts of Polygonum hydropiper L. (‘Benitade’) were analysed by capillary GC and GC–MS. Fifty-three components were identified, representing 91.6% of the total oil. The main constituents of the essential oil were (E)-β-farnesene (44.1%), phytol (10.8%), (E)-caryophyllene (9.3%), (E)-nerolidol (6.9%). The essential oil from Benitade contained a high content of sesquiterpenoids. Copyright © 2007 John Wiley & Sons, Ltd.
38 citations
TL;DR: A steam distilled oil obtained from Matteuccia struthiopteris was found to contain 103 volatile components, and (E)-phytol (24.8%), nonanal and decanal (7.6%) as the main compounds.
Abstract: A steam distilled oil obtained from Matteuccia struthiopteris was analyzed by GC and GC/MS. The oil was found to contain 103 volatile components, and (E)-phytol (24.8%), nonanal (15.1%) and decanal (7.6%) as the main compounds. The oil included two aldehydes known as sea-weed like odor, (8Z, 11Z, 14Z)-heptadecatrienal (0.6%) and (8Z, 11Z)-heptadecadienal (0.1%). The most characteristic aroma compound was (6Z)-nonenal.
33 citations
TL;DR: The compositions of the essential oil from AkeBIAE FRUCTUS and AKEBIAE CAULIS, the dried fruits and stems of Akebia quinata (THUNB.) DECNE, have been investigated and revealed the presence of 86 components, representing 98.4% of the total oil.
Abstract: The compositions of the essential oil from AKEBIAE FRUCTUS and AKEBIAE CAULIS, the dried fruits and stems of Akebia quinata (THUNB.) DECNE. (Lardizabalaceae), have been investigated by GC and GC/MS. As a result, the fruits oil was revealed the presence of 86 components, representing 98.4% of the total oil. The major compounds of the fruits oil were limonene, eugenol, octanal and p-cymene. The monoterpenoids and saturated short-chain aldehyde (C6 approximately C10) were main volatile fractions of the oil. Ninety components accounting for 90.5% of constituents of stems oil were identified, and the main compounds of the oil were hexanoic acid, palmitic acid, (2E, 4E)-decadienal and hexanol. The oil had high content of saturated fatty acids (C6 approximately C16), and unsaturated short-chain aldehyde (C6 approximately C10).
33 citations
TL;DR: CYP2A6 was the major enzyme involved in the hydroxylation of (-)-camphor by human liver microsomes, based on the following lines of evidence.
Abstract: The in vitro metabolism of (-)-camphor was examined in human liver microsomes and recombinant enzymes. Biotransformation of (-)-camphor was investigated by gas chromatography-mass spectrometry (GC-MS). (-)-Camphor was oxidized to 5-exo-hydroxyfenchone by human liver microsomal cytochrome (P450) enzymes. The formation of metabolites of (-)-camphor was determined by the relative abundance of mass fragments and retention time on gas chromatography (GC). CYP2A6 was the major enzyme involved in the hydroxylation of (-)-camphor by human liver microsomes, based on the following lines of evidence. First, of eleven recombinant human P450 enzymes tested, CYP2A6 catalyzed the oxidation of (-)-camphor. Second, oxidation of (-)-camphor was inhibited by (+)-menthofuran and anti-CYP2A6 antibody. Finally, there was a good correlation between CYP2A6 contents and (-)-camphor hydroxylation activities in liver microsomes of 9 human samples.
19 citations
TL;DR: The biotransformation of terpenoids using the plant pathogenic fungus Glomerella cingulata as a biocatalyst to produce useful novel organic compounds was investigated.
7 citations
TL;DR: Biotransformations of monoterpenoids by Spodoptera litura are reviewed and various substrates used for the transformations are included in this review of the literature for the period 1996–2006.
Abstract: Biotransformations of monoterpenoids by Spodoptera litura are reviewed. Various substrates used for the transformations are included in this review of the literature for the period 1996–2006. The m...
3 citations
TL;DR: Results suggest that human CYP2A6 and rat CyP2B1 are the major enzymes involved in the metabolism of (+)-fenchol by liver microsomes and that there are species-related differences in the human and rat CYP 2A enzymes.
Abstract: The metabolism of (+)-fenchol was investigated in vitro using liver microsomes of rats and humans and recombinant cytochrome P450 (P450 or CYP) enzymes in insect cells in which human/rat P450 and NADPH-P450 reductase cDNAs had been introduced. The biotransformation of (+)-fenchol was investigated by gas chromatography-mass spectrometry (GC-MS). (+)-Fenchol was oxidized to fenchone by human liver microsomal P450 enzymes. The formation of metabolites was determined by the relative abundance of mass fragments and retention times on GC. Several lines of evidence suggested that CYP2A6 is a major enzyme involved in the oxidation of (+)-fenchol by human liver microsomes. (+)-Fenchol oxidation activities by liver microsomes were very significantly inhibited by (+)-menthofuran, a CYP2A6 inhibitor, and anti-CYP2A6. There was a good correlation between CYP2A6 contents and (+)-fenchol oxidation activities in liver microsomes of ten human samples. Kinetic analysis showed that the Vmax/Km values for (+)-fenchol catalysed by liver microsomes of human sample HG03 were 7.25 nM-1 min-1. Human recombinant CYP2A6-catalyzed (+)-fenchol oxidation with a Vmax value of 6.96 nmol min-1 nmol-1 P450 and apparent Km value of 0.09 mM. In contrast, rat CYP2A1 did not catalyse (+)-fenchol oxidation. In the rat (+)-fenchol was oxidized to fenchone, 6-exo-hydroxyfenchol and 10-hydroxyfenchol by liver microsomes of phenobarbital-treated rats. Recombinant rat CYP2B1 catalysed (+)-fenchol oxidation. Kinetic analysis showed that the Km values for the formation of fenchone, 6-exo- hydroxyfenchol and 10-hydroxyfenchol in rats treated with phenobarbital were 0.06, 0.03 and 0.03 mM, and Vmax values were 2.94, 6.1 and 13.8 nmol min-1 nmol-1 P450, respectively. Taken collectively, the results suggest that human CYP2A6 and rat CYP2B1 are the major enzymes involved in the metabolism of (+)-fenchol by liver microsomes and that there are species-related differences in the human and rat CYP2A enzymes.
3 citations
TL;DR: The EtOAc extract from rice showed a suppressive effect on umu gene expression of the SOS response in Salmonella typhimurium TA1535/pSK1002 against the mutagen, Trp-P-1, which requires liver metabolizing enzyme.
1 citations
Patent•
23 Jan 2007
TL;DR: In this paper, a novel isolongifolen-9-one derivative can be produced by carrying out microbial conversion of isolate-on-nine-one by treating the compound with a microorganism belonging to the genus Aspergillus or Glomerella.
Abstract: PROBLEM TO BE SOLVED: To provide a novel isolongifolen-9-one derivative, preferably a novel isolongifolen-9-one derivative having tyrosinase inhibition activity, a method for producing the derivative, and a tyrosinase inhibitor containing the derivative as an active component. SOLUTION: The novel isolongifolen-9-one derivative can be produced by carrying out microbial conversion of isolongifolen-9-one by treating the compound with a microorganism belonging to the genus Aspergillus or Glomerella, and collecting the obtained isolongifolen-9-one derivative. COPYRIGHT: (C)2008,JPO&INPIT
1 citations
Patent•
23 Jan 2007
TL;DR: In this article, a novel longicyclene derivative was obtained by treating a microorganism belonging to genus Aspergillus or with its in vivo enzyme for microbial conversion of long-icyclenes and isolating the resulting long-cyclene derivative.
Abstract: PROBLEM TO BE SOLVED: To provide a novel longicyclene derivative, preferably a novel longicyclene derivative having a tyrosinase inhibition activity, and a tyrosinase-activity inhibitor comprising the derivative as an active component. SOLUTION: The novel longicyclene derivative is obtained by treating longicyclene with a microorganism belonging to genus Aspergillus or with its in vivo enzyme for microbial conversion of longicyclene and isolating the resulting longicyclene derivative. COPYRIGHT: (C)2008,JPO&INPIT