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Mohd Anuar Jonet

Researcher at Universiti Teknologi Malaysia

Publications -  9
Citations -  134

Mohd Anuar Jonet is an academic researcher from Universiti Teknologi Malaysia. The author has contributed to research in topics: Enzyme & Chemistry. The author has an hindex of 4, co-authored 5 publications receiving 101 citations.

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Optimization of a heterologous signal peptide by site-directed mutagenesis for improved secretion of recombinant proteins in Escherichia coli

TL;DR: Secretory production of other proteins, such as mannosidase, also showed similar improvement, as demonstrated by CGTase production, suggesting that the combination of an optimized SP and a suitable chemical additive leads to significant improvements of extracellular recombinant protein production and cell viability.
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Modulation of transglycosylation and improved malto-oligosaccharide synthesis by protein engineering of maltogenic amylase from Bacillus lehensis G1

TL;DR: Findings showed that modification of the active site structure imposed various effects on MAG1 activities during malto-oligosaccharide synthesis, resulting in an increase in the transglycosylation to hydrolysis ratio.
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Thermostability enhancement of xylanase Aspergillus fumigatus RT-1

TL;DR: Based on homology modelling conducted to analyze the mutants’ structures, it showed that hydrophobicity and hydrogen bonds were the driving forces that lead to the improvement of the thermal stability of these xylanase mutants.
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Optimization of a Bacillus sp signal peptide for improved recombinant protein secretion and cell viability in Escherichia coli: Is there an optimal signal peptide design?

TL;DR: Signal peptide sequence analysis and site-directed mutagenesis of G1 signal peptide demonstrated that an 'MKK' sequence in n-region and the presence of a helix-breaking residue at the centre of h-region are important elements for the design of an optimal signal peptides.