Cryopreservation of Indian red jungle fowl (Gallus gallus murghi) semen

Cryopreservation of Indian red jungle fowl (Gallus gallus murghi) semen with polyvinylpyrrolidone.

Diagnostic and Therapeutic Techniques in Animal Reproduction.

Dimethyleacetamide improves the cryosurvivability of Indian red jungle fowl (Gallus gallus murghi) sperm.

This study was designed to evaluate a range of avian semen extenders for liquid storage of Indian Red Jungle Fowl (Gallus gallus murghi) spermatozoa at 5 °C. Semen was collected from 8 mature train...

Comparison of Extenders for Liquid Storage of Indian Red Jungle Fowl (Gallus Gallus Murghi) Spermatozoa

The Indian Red Jungle Fowl is a wild native gallus subspecies of Southern Asia. Semen has never been studied in this species. In order to better know the male reproductive capacities, experiments were conducted to study the semen characteristics, impact of ejaculate collection frequencies, and timing of collection on sperm quality parameters. Mean sperm concentration 800 million/mL, total sperm per ejaculate (0.015 billion), motility (63.5 %), live/total sperm (92.4 %), intact acrosome (75.5 %), and plasma membrane integrity (89.2 %) were recorded. Percentage of abnormal sperm (head, mid-piece, and tail) was 8.1 % and recovered mainly mid-piece abnormalities. The motile sperm percentage was positively correlated with intact acrosomes (r = 0.34) and plasma membrane integrity (r = 0.41). Total sperm per ejaculate (billion) was maximum at 72 h of collection followed by 24 and 48 h of collection. Daily and weekly sperm production (billion) was found maximum at 24 h of collection compared to 12, 48, and 72 h of collection. Sperm motility was higher at 24, 48, and 72 h of collection compared to 12 h of collection, but the number of live sperm were higher at 12 h of collection compared to 24, 48, and 72 h. Sperm concentration was better in the morning time, while the values for sperm viability and plasma membrane integrity were higher in the semen collected at evening time. In conclusion, the Indian Red Jungle Fowl shows a semen production quantitatively relatively low for the species as compared to domestic chicken and contrasted parameters of quality. The semen production is affected by the frequency of collection with an optimum for a daily collection preferentially held in the evening period. These results may now be used for artificial insemination and conservation program.

Semen characteristics of the Indian Red Jungle Fowl (Gallus gallus murghi)

The effect of cysteine addition to the freezing extender on the progressive motility, viability, plasma membrane and DNA integrity of buffalo bull spermatozoa was studied. Semen from three Nili-Ravi buffalo bulls of similar age group was collected with artificial vagina. Qualifying ejaculates were split in three aliquots for dilution (50 × 106 spermatozoa ml–1) with tris-citric acid extender containing either 0.0, 0.5, 1.0 mM cysteine. Extended semen samples were cooled and equilibrated before cryopreservation. Progressive motility, plasma membrane integrity, viability and DNA integrity were assessed at 0, 2 and 4 hours post-thaw incubation (37°C). Sperm progressive motility and plasma membrane integrity of buffalo bull spermatozoa were higher (P < 0.05) in extender containing 1.0 mM cysteine than those with 0.5 mM or 0.0 mM at 0, 2 and 4 hours post-thaw. Sperm viability and DNA integrity were higher (P < 0.05) in extender containing 0.5 mM and 1.0 mM cysteine than those with 0.0 mM cysteine at 0, 2 and 4...

Effect of cysteine addition to the freezing extender on the progressive motility, viability, plasma membrane and DNA integrity of Nili-Ravi buffalo (Bubalus bubalis) bull spermatozoa

The Red Jungle Fowl (Gallus gallus murghi), a native species of Southern Asia is threatened by habitat destruction, egg collection, predation and genetic hybridisation. Semen banking is one of the ...

Muhammad Fahim Malik

Papers

Semen characteristics of the Indian Red Jungle Fowl (Gallus gallus murghi)

Effect of cysteine addition to the freezing extender on the progressive motility, viability, plasma membrane and DNA integrity of Nili-Ravi buffalo (Bubalus bubalis) bull spermatozoa

Impact of Ejaculate Frequencies on the Quality of Red Jungle Fowl (Gallus gallus murghi) semen