N
Nancy L. Courage
Publications - 7
Citations - 1976
Nancy L. Courage is an academic researcher. The author has contributed to research in topics: Fusion protein & FKBP. The author has an hindex of 7, co-authored 7 publications receiving 1890 citations.
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Journal ArticleDOI
A humanized system for pharmacologic control of gene expression
Victor M. Rivera,Tim Clackson,Sridaran Natesan,Roy Pollock,Jane F. Amara,Terence P. Keenan,Shannon R. Magari,T. B. Phillips,Nancy L. Courage,Franklin Cerasoli,Dennis A. Holt,Michael Z. Gilman +11 more
TL;DR: The properties of a suitable system for pharmacologic control of therapeutic gene expression are reported and its use to control circulating levels of human growth hormone in mice implanted with engineered human cells is demonstrated.
Journal ArticleDOI
Redesigning an FKBP–ligand interface to generate chemical dimerizers with novel specificity
Tim Clackson,Wu Yang,Leonard W. Rozamus,Marcos Hatada,Jane F. Amara,Carl T. Rollins,Lauren F. Stevenson,Shannon R. Magari,Susan A. Wood,Nancy L. Courage,Xiaode Lu,Franklin Cerasoli,Michael Z. Gilman,Dennis A. Holt +13 more
TL;DR: Ligands that bind specifically to a mutated FKBP over the wild-type protein are designed by remodeling an FK BP-ligand interface to introduce a specificity binding pocket and showed that recognition is surprisingly relaxed, with the modified ligand only partially filling the engineered cavity.
Journal ArticleDOI
Regulation of protein secretion through controlled aggregation in the endoplasmic reticulum
Victor M. Rivera,Xiurong Wang,Scott Wardwell,Nancy L. Courage,Allen Volchuk,Terence P. Keenan,Dennis A. Holt,Michael Z. Gilman,Lelio Orci,Frank Cerasoli,James E. Rothman,Tim Clackson +11 more
TL;DR: A system for direct pharmacologic control of protein secretion was developed to allow rapid and pulsatile delivery of therapeutic proteins and may make gene therapy a viable method for delivery of polypeptides that require rapid and regulated delivery.
Journal ArticleDOI
A versatile synthetic dimerizer for the regulation of protein-protein interactions.
Jane F. Amara,Tim Clackson,Victor M. Rivera,Tao Guo,Terence P. Keenan,Sridaran Natesan,Roy M. Pollock,Wu Yang,Nancy L. Courage,Dennis A. Holt,Michael Z. Gilman +10 more
TL;DR: Several families of readily prepared, totally synthetic, cell-permeable dimerizers composed of ligands for human FKBP12 proved to be exceptionally potent and versatile in all experimental contexts tested.
Journal ArticleDOI
Long-term regulated expression of growth hormone in mice after intramuscular gene transfer
Victor M. Rivera,Xuehai Ye,Nancy L. Courage,Jeff Sachar,Frank Cerasoli,James M. Wilson,Michael Z. Gilman +6 more
TL;DR: In vivo regulation of gene expression after intramuscular injection of two separate adenovirus or adeno-associated virus (AAV) vectors, one encoding an inducible human growth hormone (hGH) target gene and the other a bipartite rapamycin-regulated transcription factor are demonstrated.