Nico Stuurman

TL;DR: This unit provides step‐by‐step protocols describing how to get started working with µManager, as well as some starting points for advanced use of the software.

TL;DR: A guide to using some of the recently added advanced μManager features, including hardware synchronization, simultaneous use of multiple cameras, projection of patterned light onto a specimen, live slide mapping, imaging with multi-well plates, particle localization and tracking, and high-speed imaging.

TL;DR: The current state of knowledge on the structure, assembly, and possible functional roles of nuclear lamins is summarized, emphasizing the information concerning the ability ofnuclear lamins to self-assemble into distinct oligomers and polymers.

TL;DR: It is shown by means of a genome-wide RNA interference screen in Drosophila S2 cells that about 1.5% of all genes function in lipid-droplet formation and regulation, and a subset of the Arf1–COPI vesicular transport proteins also regulated droplet morphology and lipid utilization, thereby identifying a previously unrecognized function for this machinery.

TL;DR: Using a full-genome RNA interference screen of Drosophila S2 cells, about 200 genes that contribute to spindle assembly are identified, more than half of which were unexpected.