Nils Bergenhem

TL;DR: Prolyl isomerase, which has been shown to accelerate rate-limiting cis-trans peptidyl-proline isomerization steps in the folding pathway, can also participate in the protein-folding process as a chaperone.

TL;DR: Members of the a-carbonic anhydrase (α-CA) gene family encode not only proteins that exhibit the characteristic catalytic activity of CA, but also CA-related proteins that are apparently devoid of this activity.

TL;DR: The magnitude of Kcat, its pH profile, 18O-exchange data for both wild-type and a H64A mutant, and inhibition by CuSO4 and acrolein suggest that the histidine at position 64 is functioning as a proton-transfer group and is responsible for one of the observed ionizations, which suggests a role for the amino-terminal end in enhancing proton transfer in catalysis by carbonic anhydrase.

TL;DR: The polymerase chain reaction (PCR) is used to amplify human CARP sequences from several cDNA libraries and the sequence has an 89.3% sequence identity with mouse CARP at the nucleotide level and 97.9% at the amino acid level.

TL;DR: The data suggest that the core of theprotein undergoes continued structural rearrangements affecting the rigidity of the protein environment surrounding the emitting tryptophan and the protein lability long after the return of enzyme activity.