N
Nina Järvinen
Researcher at University of Helsinki
Publications - 6
Citations - 243
Nina Järvinen is an academic researcher from University of Helsinki. The author has contributed to research in topics: Fucose & Fucosyltransferase. The author has an hindex of 5, co-authored 6 publications receiving 234 citations.
Papers
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Journal ArticleDOI
Analysis of nucleotide sugars from cell lysates by ion-pair solid-phase extraction and reversed-phase high-performance liquid chromatography.
Jarkko Räbinä,Minna Mäki,Emma M. Savilahti,Nina Järvinen,Leena Penttilä,Risto Renkonen,Risto Renkonen +6 more
TL;DR: Practical methods for both extraction of nucleotide sugars from cell lysates and for their analytical separation enable good separation of structurally similar sugar nucleotides, compatibility with rapid evaporative concentration, and possibility to automation.
Journal ArticleDOI
Cloning and expression of Helicobacter pylori GDP‐l‐fucose synthesizing enzymes (GMD and GMER) in Saccharomyces cerevisiae
TL;DR: Cloned the two key enzymes of GDP-l-fucose synthesis, H. pylori gmd coding for GDP-d-mannose dehydratase (GMD), and gmer coding for GMER, and expressed them in an enzymatically active form in Saccharomyces cerevisiae and the end product of these enzymes was used as a fucose donor in an fucosyltransferase assay.
Journal ArticleDOI
Functional expression of Pseudomonas aeruginosa GDP-4-keto-6-deoxy-D-mannose reductase which synthesizes GDP-rhamnose.
TL;DR: The enzymatic characterization of P. aeruginosa RMD expressed in Saccharomyces cerevisiae is described, which confirmed to be GDP-rhamnose by HPLC, matrix-assisted laser desorption/ionization time-of-flight MS, and finally NMR spectroscopy.
Journal ArticleDOI
Cloning and functional expression of a novel GDP-6-deoxy-D-talose synthetase from Actinobacillus actinomycetemcomitans.
TL;DR: The functional expression of gts provides another enzymatically defined pathway for the synthesis of GDP-deoxyhexoses, which can be used as donors for the corresponding glycosyltransferases.
Patent
Use of recombinant enzymes for preparing GDP-L-fucose and fucosylated glycans
Laura Hirvas,Solveig Hortling,Nina Järvinen,Tuula Kallioinen,Sirkka-Liisa Kauranen,Minna Mäki,Pirkko Mattila,Jaana Niittymäki,Jarkko Räbinä,Risto Renkonen +9 more
TL;DR: In this paper, the use of recombinant enzymes for the preparation of GDP-L-fucose and fucosylated glycans is disclosed, and means useful in the process are provided, including enzymes, chimeric enzymes, DNA sequences, genes, vectors and host cells.