Octavio T. Ramírez

TL;DR: In this review, the theoretical analyses that predict the existence of environmental gradients in bioreactors and their experimental confirmation are reviewed and studies based on the scale-down methodology, a convenient tool for assessing the effect of environmental heterogene ities, are surveyed.

TL;DR: This chapter discusses common problems in recombinant protein production, which include molecular biology techniques, as well as manipulation of the culture environment, and strategies for their solution.

TL;DR: Due to the lack of catabolite repression, PB12 strain carrying multicopy plasmids containing tktA and aroGfbrgenes is capable of coutilizing glucose and other carbon sources; this capacity, reduces its μ but increases the production of aromatic compounds.

TL;DR: The current status of the heat inducible expression system based on the pL and/or pR λ phage promoters is described, focusing on recent developments on expression vehicles, the stress responses at the molecular and physiological level that occur after heat induction, and bioprocessing factors that affect protein overexpression, including culture operation variables and induction strategies.

TL;DR: It is proposed that in addition to the glycolytic metabolism, a gluconeogenic carbon recycling process that involves acetate is occurring simultaneously in this strain when growing on glycerol.