Francisco Bolívar

TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).

TL;DR: In vitro recombination via restriction endonucleases and the in vivo genetic translocation of the Ap resistance (Apr) gene resulted in the construction of a new cloning vehicle, the plasmid pBR313, which has a molecular weight of 5.8 Mdalton and has been characterized using thirteen restriction enzymes, six of which cleave the plasid at unique restriction sites.

TL;DR: This work has reported the first synthesis of a functional polypeptide product from a gene of chemically synthesized origin, including the sequence of amino acids corresponding to somatostatin, in vitro.

TL;DR: Synthetic genes for human insulin A and B chains were cloned separately in plasmid pBR322 and fused to an Escherichia coli beta-galactosidase gene to provide efficient transcription and translation and a stable precursor protein.

TL;DR: In vitro recombinant DNA techniques were used to construct two new cloning vehicles, pBR324 and pBR235, which permit the molecular cloning and easy selection of EcoRI, BamHI, HindIII, PstI, HincII, SalI, (XamI), Smal, ( XmaI), BglII and DpnII restriction generated DNA molecules.