Showing papers by "Olli Kallioniemi published in 1992"
TL;DR: Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome, which identified 16 different regions of amplification, many in loci not previously known to be amplified.
Abstract: Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome. Differentially labeled test DNA and normal reference DNA are hybridized simultaneously to normal chromosome spreads. The hybridization is detected with two different fluorochromes. Regions of gain or loss of DNA sequences, such as deletions, duplications, or amplifications, are seen as changes in the ratio of the intensities of the two fluorochromes along the target chromosomes. Analysis of tumor cell lines and primary bladder tumors identified 16 different regions of amplification, many in loci not previously known to be amplified.
3,413 citations
Journal Article•
TL;DR: The data suggest that the dominant mechanism of allelic loss at 17p in breast cancer is a physical deletion and that analysis of deletions by fluorescence in situ hybridization is a rapid and sensitive approach to studying chromosomal aberrations.
Abstract: Allelic loss of tumor suppressor genes on chromosome 17p has been implicated in the progression of breast cancer. This is in principle detectable by fluorescence in situ hybridization if the loss occurs by deletion. In order to determine if detectable deletions occur in primary breast cancer, we used dual-color hybridization with chromosome 17 pericentromeric and region-specific DNA probes to study 19 primary breast cancers. The copy numbers of 17 centromere and 17p13.1 sequences were compared with the loss of heterozygosity (LOH) for probe YNZ22 at 17p13.3 detected by restriction fragment length polymorphism. Nine of 11 cases showing LOH also showed the major population of nuclei with a deletion. The remaining two tumors with LOH were trisomic for both the centromere and 17p13.1 cosmid. In contrast, seven of eight tumors without LOH had no deletions by fluorescence in situ hybridization. These data suggest that the dominant mechanism of allelic loss at 17p in breast cancer is a physical deletion and that analysis of deletions by fluorescence in situ hybridization is a rapid and sensitive approach to studying chromosomal aberrations.
108 citations
Journal Article•
TL;DR: Patients who had either partially or uniformly EGFR-positive carcinomas had a worse 10-yr progression-free, overall, and prostatic carcinoma-specific survival than those with EG FR-negative carcinomas, and according to a multivariate analysis, EGFR did not have independent prognostic value.
99 citations
TL;DR: Analysis of clinical breast cancer samples showed that most of the cells contained two copies of the RB1 gene, even when restriction fragment length polymorphism analysis showed loss of heterozygosity (LOH) at theRB1 locus, which indicates that LOH at the RB 1 locus in breast cancer cells probably involves mechanisms other than physical deletion.
Abstract: Fluorescence in situ hybridization (FISH) was applied to detect the copy number of the retinoblastoma (RB1) tumor suppressor gene in metaphase chromosomes and interphase nuclei. We used 14 λ phage clo
50 citations
TL;DR: The techniques of fluorescence in situ hybridization, primed in situ labeling and comparative genome hybridization are described, as are probes for repeated sequences, whole chromosomes and specific loci.
26 citations
TL;DR: The data do not suggest that flow cytometric analysis of paraffin sections as a grading method would be more consistent than quantitative histopathology from sections, especially when compared with subjective histological grading.
Abstract: 116 paraffin-embedded breast cancer samples were analyzed by flow cytometry From each sample 3 consecutive 50 microns sections were cut for the study The presence of neoplastic tissue was verified from light microscope sections cut before and after the adjacent sections One laboratory started with one section from each block and was allowed extra sections when needed for analysis At the end the laboratory obtained results from all 116 cases The rest of the samples were studied by 2 other laboratories Samples with results from 2 or 3 laboratories then allowed variability analysis and the estimation of the grading efficiency in a 2-grade system Inconsistency in diploidy/aneuploidy distinction was present in 36 of 111 (324%) cases studied by two or three laboratories This inconsistency was less obvious in samples graded as multiploid by at least one of the laboratories The grading efficiency as analyzed from the results of 3 laboratories was 089, and of 2 laboratories, 084 The DNA index showed a slightly higher grading efficiency At the cutoff point of 13, 91% of cases could be expected to be correctly classified into low ploidy and high ploidy groups (grading efficiency 091) The S-phase fraction had a mean grading efficiency of 089, a performance comparable to that of diploidy/aneuploidy distinction In the light of the available data the flow cytometric analysis can add to the consistency of grading, especially when compared with subjective histological grading However, the data do not suggest that flow cytometric analysis of paraffin sections as a grading method would be more consistent than quantitative histopathology from sections
13 citations