Journal ArticleDOI
Comparative Genomic Hybridization for Molecular Cytogenetic Analysis of Solid Tumors
Anne Kallioniemi,Olli Kallioniemi,Damir Sudar,Denis Rutovitz,Joe W. Gray,Fred Waldman,Daniel Pinkel +6 more
TLDR
Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome, which identified 16 different regions of amplification, many in loci not previously known to be amplified.Abstract:
Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome. Differentially labeled test DNA and normal reference DNA are hybridized simultaneously to normal chromosome spreads. The hybridization is detected with two different fluorochromes. Regions of gain or loss of DNA sequences, such as deletions, duplications, or amplifications, are seen as changes in the ratio of the intensities of the two fluorochromes along the target chromosomes. Analysis of tumor cell lines and primary bladder tumors identified 16 different regions of amplification, many in loci not previously known to be amplified.read more
Citations
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Journal ArticleDOI
Relative quantification of 40 nucleic acid sequences by multiplex ligation-dependent probe amplification
Jan P. Schouten,Cathal J. McElgunn,Raymond Waaijer,Danny A. Zwijnenburg,Filip Diepvens,Gerard Pals +5 more
TL;DR: A new method for relative quantification of 40 different DNA sequences in an easy to perform reaction requiring only 20 ng of human DNA is described.
Journal ArticleDOI
Cell cycle control and cancer
TL;DR: New insights in understanding of the cell cycle reveal how fidelity is normally achieved by the coordinated activity of cyclin-dependent kinases, checkpoint controls, and repair pathways and how this fidelity can be abrogated by specific genetic changes.
Journal ArticleDOI
A multigenic program mediating breast cancer metastasis to bone.
Yibin Kang,Peter M. Siegel,Weiping Shu,Maria Drobnjak,Sanna M. Kakonen,Carlos Cordon-Cardo,Theresa A. Guise,Theresa A. Guise,Joan Massagué +8 more
TL;DR: Overexpression of this bone metastasis gene set is superimposed on a poor-prognosis gene expression signature already present in the parental breast cancer population, suggesting that metastasis requires a set of functions beyond those underlying the emergence of the primary tumor.
Journal ArticleDOI
High resolution analysis of DNA copy number variation using comparative genomic hybridization to microarrays
Daniel Pinkel,Daniel Pinkel,Richard Segraves,Damir Sudar,Steven M. Clark,Ian Poole,David Kowbel,Colin Collins,Wen Lin Kuo,Chira Chen,Ye Zhai,Shanaz H. Dairkee,Britt-Marie Ljung,Joe W. Gray,Joe W. Gray,Donna G. Albertson,Donna G. Albertson,Donna G. Albertson +17 more
TL;DR: The implementation of array CGH is demonstrated to be able to measure copy number with high precision in the human genome, and to analyse clinical specimens by obtaining new information on chromosome 20 aberrations in breast cancer.
Journal ArticleDOI
Circular binary segmentation for the analysis of array-based DNA copy number data.
TL;DR: A modification ofbinary segmentation is developed, which is called circular binary segmentation, to translate noisy intensity measurements into regions of equal copy number in DNA sequence copy number.
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