P
Pal Maliga
Researcher at Rutgers University
Publications - 207
Citations - 18181
Pal Maliga is an academic researcher from Rutgers University. The author has contributed to research in topics: Plastid & Gene. The author has an hindex of 70, co-authored 203 publications receiving 17614 citations. Previous affiliations of Pal Maliga include Hungarian Academy of Sciences.
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Journal ArticleDOI
Amplification of a Chimeric Bacillus Gene in Chloroplasts Leads to an Extraordinary Level of an Insecticidal Protein in Tobacco
TL;DR: It is reported that amplification of an unmodified crylA(c) coding sequence in chloroplasts up to ∼ 10,000 copies per cell resulted in the accumulation of an unprecedented 3–5% of the soluble protein hi tobacco haves as protoxin.
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Progress towards commercialization of plastid transformation technology
TL;DR: Tobacco chloroplasts are ready to be tested as a platform for the expression of recombinant proteins on a commercial scale with the promise of reproducible yields of 5-25% of total soluble cellular protein in leaves and reliability has been achieved through refinement of an expression toolkit.
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Stable genetic transformation of intact Nicotiana cells by the particle bombardment process
TL;DR: The results show that gene transfer by high-velocity microprojectiles is a rapid and direct means for transforming intact plant cells and tissues that eliminates the need for production of protoplasts or infection by Agrobacterium.
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Fluorescent antibiotic resistance marker for tracking plastid transformation in higher plants.
Muhammad Sarwar Khan,Pal Maliga +1 more
TL;DR: The report reports a system of marker genes for plastid transformation, termed FLARE-S, which is obtained by translationally fusing aminoglycoside 3"-adenyltransferase with the Aequorea victoria green fluorescent protein and confers resistance to both spectinomycin and streptomycin.
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Interspecific chloroplast recombination in a Nicotiana somatic hybrid
TL;DR: In the Pt14 chloroplast genome three N. tabacum and four N. plumbaginifolia parent specific restriction sites have been identified, indicating that the pt14 chloroplasts genome contains at least six recombination sites.