Pal Maliga

TL;DR: A plastid transformation approach was assessed in order to produce a plant-based HPV-16 L1 vaccine and formation of immunogenic VLPs in chloroplasts was confirmed by capture ELISA assay using antibodies recognizing conformational epitopes and by electron microscopy.

TL;DR: The isolation of an isoleucine-requiring line from haploid cell cultures of Nicotiana plumbaginifolia and the regeneration of diploid plants with no detectable activity of L-threonine deaminase (EC 4.2.1.16), the first enzyme in isoleUCine biosynthesis.

TL;DR: Examination of plastid marker genes by the phiC31 phage site-specific integrase (Int) that mediates recombination between bacterial (attB) and phage (attP) attachment sites expands the repertoire of the tools available for the manipulation of the plastsid genome.

TL;DR: Previous reports in Antirrhinum majus, Epilobium hirsutum, Nicotiana tabacum, Petunia hybrida, and the cereal crop Setaria italica are extended to a cruciferous species suggesting that low-frequency paternal leakage of plastids via pollen may be universal in plants previously thought to exhibit strict maternal plastid inheritance.

TL;DR: Evidence is presented for cell-to-cell movement of the entire 161-kb plastid genome in these plants, most likely in intact plastids, which supports the universality of intercellular organelle trafficking and may enable development of future biotechnological applications.