Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction

This book is one of our contemporary medical bibles. It needs no introduction; those who have frequent need of it know it very well, and those who use it less often seek it out on the library shelf when problems arise. The book is truly encyclopedic. Everything relevant discovered during the six-year intervals between publication finds its way into these pages. By today's standards, its 1,778 closely packed small-print pages are a bargain. The mutant gene is both hero and villain in this book. It is reponsible for the biochemical abnormality that results in disease, no matter how rare a given abnormality may be. By the same token, however, it is truly an experiment of nature, shedding light on fundamental metabolic sequences and biologic mechanisms. This volume, more than most, explains the contributions of the laboratory to clinical medicine. Each chapter seems to have been rewritten, so that the exciting

The Metabolic Basis Of Inherited Disease.

OBJECTIVE To establish an indirect immunofluorescent test so as to improve the sensitivity and specificity of examination of antibodies to beta(2)-glycoprotein METHODS Full-length beta(2)GP cDNA was obtained from human hepatocellular cancer cell line HepG2 by RT-PCR and cloned into the mammalian expression vector pEGFP-C1 The recombinant plasmid pEGFP-beta(2)GP was transfected into HEp-2 cells RT-PCR, immunoblotting (IBT), confocal fluorescence microscopy, and indirect immunofluorescent test (IIF) were used to confirm the expression, localization, and antigenicity of fusion protein of green fluorescent protein (GFP) Serum specimens from 19 patients suspected as with secondary antiphospholipid syndrome (APS), 1 patient diagnosed as with primary APS, and 10 normal persons were detected with IIF-IgG-beta(2)GP1, ELISA-IgG-ACL, and ELISA-IgG-beta(2)GP I simultaneously RESULTS (1) The HEp-beta(2)GP I cells thus obtained retained their ability of expression of beta(2)GP-I-GFP for more than ten generations This beta(2)GP-I-GFP showed the antigenicity of beta(2)GP-I with a characteristic feature (2) Seven of the 20 serum specimens from APS patients showed characteristic immunofluorescent pattern No serum specimen from normal persons showed immunofluorescent staining The comparison of results of the three methods showed that the concordance between IIF-IgG-beta(2)GP I and ELISA-IgG-beta(2)GP I was the most perfect (Kappa = 0886) (3) HEp-beta(2)GP I retained the immunofluorescent property of HEp-2 cell CONCLUSION As a new kind of substrate of IIF, beta(2)GP I transfectant can be used to detect anti-beta(2)GP-I antibodies Transfeted HEp-2 cells keep the immunofluorescent property of HEp-2 cells in IFANA test and can be used as substrate for routine IFANA detection

[Expression of fusion proteins in beta(2)GP I gene-transfected HEp-2 cells and its clinical application].

Tamoxifen for early breast cancer: An overview of the randomised trials

Diabetic cardiomyopathy: Mechanisms and new treatment strategies targeting antioxidant signaling pathways

Immunomodulatory effects of Semecarpus anacardium LINN. nut milk extract (SA) were investigated in adjuvant induced arthritis by studying the alterations in humoral and cell mediated immune responses and also the anti-inflammatory effects by evaluating the changes in paw edema, tumour necrosis factor (TNF-α), nitric oxide and myeloperoxidase activities. Pharmacological studies were also conducted with SA and indomethacin on experimental animals for evaluating the anti-inflammatory, analgesic, antipyretic and ulcerogenic activities. The alterations in the humoral and cell mediated immunity were significantly reverted back to near normal levels on treatment with SA. The drug significantly reduced the elevation in the paw edema, TNF-α, nitric oxide and myeloperoxidase levels when compared with adjuvant induced arthritic animals, which shows the anti-inflammatory activity of the drug. SA showed strong anti-inflammatory effects in xylene-induced ear edema and formalin-induced inflammation. In analgesic test, the extract elicited a potential activity on both acetic acid-induced writhing response as well as hot plate test showing its central and peripheral mediated action. The drug also elicited antipyretic action in yeast-induced hyperemia in rats. In addition, the extract did not produce any ulceration on gastric mucosa during ulcerogenic test and did not produce any serious adverse effects. All these effects are nearly similar to the activities of indomethacin except the ulceration where indomethacin produced significant ulceration. From this study, the protective immunological and pharmacological role of SA is demonstrated.

Immunomodulatory and anti-inflammatory effects of Semecarpus anacardium LINN. Nut milk extract in experimental inflammatory conditions.

Kalpaamruthaa (KA), a modified indigenous Siddha formulation constitutes Semecarpus anacardium nut milk extract, Emblica officinalis and honey. KA is evaluated for its behavioral and toxicological effects and also its consequence on biochemical and histological variations. Acute and subacute toxicity studies with KA were done on Wistar Albino rats. During acute toxicity study (72 hr), there were no any adverse effects found in the general behavior and mortality at any dose level given (50–2000 mg/kg b.wt.). In subacute toxicity study (30 days) KA (50, 100, 250 and 500 mg/kg b.wt.) did not cause any changes in hematological and biochemical parameters with the exception of a transient rise in hemoglobin, leukocyte count, free fatty acid, plasma and urine creatinine and a significant decrease in blood glucose, total cholesterol, triglyceride and phospholipid levels. The changes observed are significant only at the highest dosage of 500 mg/kg b.wt. Further, histopathological examination of vital organs showed normal architecture suggesting no morphological disturbances; it can be considered that KA is safe and non toxic.

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Palanivelu Shanthi

Papers

Immunomodulatory and anti-inflammatory effects of Semecarpus anacardium LINN. Nut milk extract in experimental inflammatory conditions.

Oral Acute and Subacute Toxicity Studies with Kalpaamruthaa, a Modified Indigenous Preparation, on Rats

Effect of tangeretin, a polymethoxylated flavone on glucose metabolism in streptozotocin-induced diabetic rats.

Evaluation of antioxidant effect of Semecarpus anacardium Linn. nut extract on the components of immune system in adjuvant arthritis.

Modulatory effect of green tea extract on hepatic key enzymes of glucose metabolism in streptozotocin and high fat diet induced diabetic rats.