https://www.nhlcyberfamily.org/tests/gene%20profiling%20diffuse.pdf

Confirmation of the molecular classification of diffuse large B-cell lymphoma by immunohistochemistry using a tissue microarray

Hodgkindisease (HD) is characterized by a small numberofputativemalignant cells [HodgkinandReed-Sternberg (HRS) cells] among a background of lymphocytesandhistiocytes. The lineage ofHRS cells is still elusive anda clonal origin of these rare cells hasnot formally been demon-strated. Weisolated HRScells by micromanipulation fromhistologicalsectionsofthreecasesofHodgkinlymphoma(eachrepresenting a distinct subtype of the disease) and analyzedindividual cells for immunoglobulin variable (V) gene rear- rangements by PCR. In each of the three cases a singieheavy-chain V (VH) (and in one case, in addition, a K light-chain)generearrangement was amplifiedfromtheHRScells, identifying these cells as membersof a single done. A poten-tiallyfunctionalVHrearrangement was obtainedfrom acase ofnodular sclerosis HD. Somatic mutations and intraclonal di- versity in the VHgenesindicate a germinal center B-cell originof the HRScells in a case of lymphocyte-predominant HD,whereas ina caseof mixed-cellularity

Hodgkin disease: Hodgkin andReed-Sternberg cells picked from histological sections showclonal immunoglobulin gene rearrangements andappear tobederived fromB cells atvarious stages ofdevelopment

https://www.hemonc.theclinics.com/article/S0889-8588(14)00047-1/pdf

Anemia of inflammation

Background Merkel cell carcinoma (MCC) is a rare, aggressive skin cancer. Objective We sought to describe primary MCC incidence trends, epidemiology, and predictors of survival. Methods The population covered by the Surveillance, Epidemiology, and End Results Program was analyzed as a prospective cohort. We measured age-adjusted incidence rates (per 100,000 person-years) and effect of age, anatomic site, and stage on survival. Results Incidence was higher in males (0.34) than in females (0.17). Cases (n = 1034) occurred mostly in whites (94%), in people older than 65 years (76%), and at the head (48%). The 5-year relative survival was 75%, 59%, and 25% for localized, regional, and distant MCC, respectively. Female sex, limb presentation, localized disease, and younger age were positive predictors of survival. Conclusion The highest incidence of MCC was observed in whites, males, and in people older than 65 years. Only 49% of cases were reported as localized. Better survival was associated with limb localization, early-stage disease, younger age, and female sex.

https://www.jaad.org/article/S0190-9622(03)02108-X/pdf

Epidemiology of primary Merkel cell carcinoma in the United States

Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. It is diagnosed when serum iron concentrations are low despite adequate iron stores, as evidenced by serum ferritin that is not low. In the setting of inflammation, it may be difficult to differentiate AI from iron deficiency anemia, and the 2 conditions may coexist. Treatment should focus on the underlying disease. Recent advances in molecular understanding of AI are stimulating the development of new pathophysiologically targeted experimental therapies.

The KIT receptor tyrosine kinase (CD117 antigen) is found in a variety of normal tissue cell types and in several malignant tumors, including acute myeloid leukemia. We recently encountered two tumors initially suspected as acute myeloid leukemia cutis and expressing CD117 that showed punctate positivity for cytokeratin 20 diagnostic for Merkel cell carcinoma. We evaluated 20 additional cases of MCC and found that 21 of 22 tumors (95%) expressed CD117. Intensity of CD117 expression did not appear to correlate with aggressive behavior. While the function of the KIT receptor in MCC is not known, its pathogenic role in other malignant neoplasms suggests the possibility of a similar role in MCC.

CD117 (KIT receptor) expression in Merkel cell carcinoma.

The clinical significance and prognostic value of CD10 in de novo diffuse large B-cell lymphoma (DLBCL) is largely unknown. We retrospectively studied 19 men and 9 women based on the following criteria: (1) DLBCL with no evidence of concomitant or antecedent follicular lymphoma; (2) available flow cytometric immunophenotyping data, including CD10 status; (3) older than 15 years; (4) specific exclusion of high-grade, Burkitt-like lymphoma; and (5) exclusion of primary cutaneous DLBCL. When available, clinical data at diagnosis, including components of the international prognostic index, were reviewed. Eleven cases were CD10+, and 17 were CD10-. There was no significant difference between the CD10+ and CD10- groups in age, sex, stage, performance status, extranodal involvement, or serum lactate dehydrogenase levels at diagnosis. However, in the 26 cases for which follow-up data were available, the CD10+ group displayed a shorter overall survival than the CD10- group (8 vs 30 months). Although the clinical findings at diagnosis are similar in CD10+ and CD10- DLBCL, CD10 expression is associated with shortened overall survival. Therefore, our data suggest CD10 expression may have prognostic importance in adults with de novo DLBCL.

The clinical significance of CD10 antigen expression in diffuse large B-cell lymphoma.

Peripheral T-Cell Lymphoma Mimicking Marginal Zone B-Cell Lymphoma

In our experience, certain commonly cited immunophenotypic features of nodular lymphocyte predominant Hodgkin lymphoma (NLPHL) are not encountered in day-to-day practice. We reviewed 60 cases of NLPHL (18 women, 42 men; median age, 34 years) to discern immunophenotypic features from a large, single-institution cohort. All cases contained lymphocytic and histiocytic (LH 100% of cases (55/55) demonstrated CD3+ rosettes. Although CD57+ T cells were common within the background infiltrate, CD57+ rosettes were seen in only 48% of cases (15/31) and were rare when encountered. Based on these patterns, we conclude that bcl-2 and bcl-6 may be useful additions to the immunophenotypic analysis of NLPHL, but that the diagnostic usefulness of epithelial membrane antigen and CD57 rosettes may have been overemphasized in previous reports.

/pdf/nodular-lymphocyte-predominant-hodgkin-lymphoma-an-5181sm1o1a.pdf

Nodular lymphocyte predominant Hodgkin lymphoma. An immunophenotypic reappraisal based on a single-institution experience

http://ajcp.oxfordjournals.org/content/ajcpath/119/2/192.full.pdf

Patricia Uherova

Papers

CD117 (KIT receptor) expression in Merkel cell carcinoma.

The clinical significance of CD10 antigen expression in diffuse large B-cell lymphoma.

Peripheral T-Cell Lymphoma Mimicking Marginal Zone B-Cell Lymphoma

Nodular lymphocyte predominant Hodgkin lymphoma. An immunophenotypic reappraisal based on a single-institution experience

Nodular Lymphocyte Predominant Hodgkin Lymphoma