Showing papers by "Peter J. Rossky published in 2021"
12 citations
TL;DR: In this paper, the authors examined antibody conformational changes and how they perturb antibody motion and affect ensembles' elution profiles during ion exchange chromatography (IEX) and showed that the absence of salt enhances electrostatic attraction between the stationary phase and the immunoglobulin G (IgG).
Abstract: Conformational changes of antibodies and other biologics can decrease the effectiveness of pharmaceutical separations. Hence, a detailed mechanistic picture of antibody-stationary phase interactions that occur during ion-exchange chromatography (IEX) can provide critical insights. This work examines antibody conformational changes and how they perturb antibody motion and affect ensemble elution profiles. We combine IEX, three-dimensional single-protein tracking, and circular dichroism spectroscopy to investigate conformational changes of a model antibody, immunoglobulin G (IgG), as it interacts with the stationary phase as a function of salt conditions. The results indicate that the absence of salt enhances electrostatic attraction between IgG and the stationary phase, promotes surface-induced unfolding, slows IgG motion, and decreases elution from the column. Our results reveal previously unreported details of antibody structural changes and their influence on macroscale elution profiles.
4 citations