P
Peter M. Warnecke
Researcher at Royal Prince Alfred Hospital
Publications - 9
Citations - 1420
Peter M. Warnecke is an academic researcher from Royal Prince Alfred Hospital. The author has contributed to research in topics: DNA methylation & Methylation. The author has an hindex of 9, co-authored 9 publications receiving 1379 citations. Previous affiliations of Peter M. Warnecke include University of Sydney.
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Journal ArticleDOI
Detection and measurement of PCR bias in quantitative methylation analysis of bisulphite-treated DNA
Peter M. Warnecke,Clare Stirzaker,John R. Melki,Douglas Spencer Millar,Cheryl L. Paul,Susan J. Clark,Susan J. Clark +6 more
TL;DR: This study presents a simple method for detection and measurement of PCR bias for any set of primers, and investigates parameters for overcoming PCR bias.
Journal ArticleDOI
Identification and resolution of artifacts in bisulfite sequencing
Peter M. Warnecke,Clare Stirzaker,Jenny Z. Song,Christoph Grunau,John R. Melki,Susan J. Clark,Susan J. Clark,Susan J. Clark +7 more
TL;DR: This article focuses on conditions that inhibit complete bisulfite-mediated conversion of cytosines in a target sequence, and demonstrates the necessity of complete protein removal from DNA samples prior to bisulfITE treatment.
Journal Article
Extensive DNA Methylation Spanning the Rb Promoter in Retinoblastoma Tumors
Clare Stirzaker,Douglas Spencer Millar,Cheryl L. Paul,Peter M. Warnecke,Janet Harrison,Paul C. Vincent,Marianne Frommer,Susan J. Clark +7 more
TL;DR: The dynamics of DNA methylation in cancer cells are clearly different from normal cells and an insight into the mechanism of abnormal methylation of CpG islands incancer cells is given.
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Bisulfite sequencing in preimplantation embryos: DNA methylation profile of the upstream region of the mouse imprinted H19 gene
TL;DR: A modification of the bisulfite genomic sequencing protocol that enables detection of methylation from as few as five diploid cells from preimplantation mouse embryos is described and it is suggested that it is the overall level ofmethylation that is responsible for maintenance of the imprinting element and not the methylation of individual CpG sites.
Journal ArticleDOI
Increased DNA methyltransferase expression in leukaemia
TL;DR: The results support but do not prove the hypothesis that an increase in DNA methyltransferase activity is associated with malignant haematological diseases and may constitute a key step in carcinogenesis.