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Principles Of Fluorescence Spectroscopy

https://research.birmingham.ac.uk/portal/files/40126586/Carstea_et_al_in_press.pdf

Fluorescence spectroscopy for wastewater monitoring: a review

Understanding the processes that are driving variation of natural microbial communities across space or time is a major challenge for ecologists. Environmental conditions strongly shape the metabolic function of microbial communities; however, other processes such as biotic interactions, random demographic drift or dispersal limitation may also influence community dynamics. The relative importance of these processes and their effects on community function remain largely unknown. To address this uncertainty, here we examined bacterial and archaeal communities in replicate 'miniature' aquatic ecosystems contained within the foliage of wild bromeliads. We used marker gene sequencing to infer the taxonomic composition within nine metabolic functional groups, and shotgun environmental DNA sequencing to estimate the relative abundances of these groups. We found that all of the bromeliads exhibited remarkably similar functional community structures, but that the taxonomic composition within individual functional groups was highly variable. Furthermore, using statistical analyses, we found that non-neutral processes, including environmental filtering and potentially biotic interactions, at least partly shaped the composition within functional groups and were more important than spatial dispersal limitation and demographic drift. Hence both the functional structure and taxonomic composition within functional groups of natural microbial communities may be shaped by non-neutral and roughly separate processes.

High taxonomic variability despite stable functional structure across microbial communities

Environmental chemistry of organosiloxanes.

The link between composition and reactivity of dissolved organic matter (DOM) is central to understanding the role aquatic systems play in the global carbon cycle; yet, unifying concepts driving mo

Unifying Concepts Linking Dissolved Organic Matter Composition to Persistence in Aquatic Ecosystems.

An online repository of published organic fluorescence spectra has been developed, which can be searched for quantitative matches with any set of unknown spectra. It fills a critical gap by increasing access to measured and modelled (PARAFAC) spectra, and linking across studies and systems to reveal "global" fluorescence trends.

/pdf/openfluor-an-online-spectral-library-of-auto-fluorescence-by-3nf29lpp3k.pdf

OpenFluor- an online spectral library of auto-fluorescence by organic compounds in the environment

The spectra responsible for natural dissolved organic matter (DOM) fluorescence in 90 peer-reviewed studies have been compared using new similarity metrics. Numerous spectra cluster in specific wavelength regions. The emerging patterns suggest that most fluorescence spectra are not tied to biogeochemical origin, but exist across a wide range of different environments.

https://pubs.rsc.org/en/content/articlepdf/2019/ay/c8ay02422g

Philip Wenig

Papers

OpenFluor- an online spectral library of auto-fluorescence by organic compounds in the environment

Emerging patterns in the global distribution of dissolved organic matter fluorescence

Characterizing odorous emissions using new software for identifying peaks in chemometric models of gas chromatography-mass spectrometry datasets

Epistemic uncertainty in URANS based CFD analysis of buoyancy driven flows—Comparison of URANS and LES

Cfd Uncertainty Quantification Using Stochastic Spectral Methods - Exemplary Application to a Buoyancy-Driven Mixing Process