Philippe Margot

TL;DR: The regulatory unit of Bacillus subtilis strain 168 encompassing the structural genes of the N-acetylmuramoyl-L-alanine amidase and of its modifier has been sequenced, and found to be a divergon consisting of divergently transcribed operons lytABC and lytR.

TL;DR: Analysis of degradation products of cell wall devoid of teichoic-acid-bound O-esterified D-alanine unambiguously confirmed that LytF cuts the γ-D-glutamate-meso-diaminopimelate bond.

TL;DR: The nucleotide sequence of wprA, a protease-encoding gene of Bacillus subtilis 168, is reported and sequence analyses and enzymic assays reveal that CWBP52 is a serine protease.

TL;DR: Zymographs unambiguously reveal that the absence of CWBP33, due to inactivation of lytE, is accompanied by the loss of a lytic activity, although autolysis of corresponding, purified cell walls does not seem to be affected.

TL;DR: Insertional mutagenesis, using integrational plasmids carrying relevant fragments from the tag region, provides strong evidence that biosynthesis of polyglycerol phosphate [poly(groP], so far largely considered as a dispensable polymer, is in fact essential for growth.