P
Pichet Praveschotinunt
Researcher at Wyss Institute for Biologically Inspired Engineering
Publications - 13
Citations - 635
Pichet Praveschotinunt is an academic researcher from Wyss Institute for Biologically Inspired Engineering. The author has contributed to research in topics: Self-healing hydrogels & Escherichia coli. The author has an hindex of 6, co-authored 13 publications receiving 335 citations. Previous affiliations of Pichet Praveschotinunt include Harvard University.
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Journal ArticleDOI
Engineered Living Materials: Prospects and Challenges for Using Biological Systems to Direct the Assembly of Smart Materials.
Peter Q. Nguyen,Noémie-Manuelle Dorval Courchesne,Anna M. Duraj-Thatte,Pichet Praveschotinunt,Neel Joshi +4 more
TL;DR: Early efforts toward engineered living materials (ELMs) are reviewed, with an emphasis on engineered bacterial systems, living composite materials which integrate inorganic components, successful examples of large-scale implementation, and production methods.
Journal ArticleDOI
Engineered E. coli Nissle 1917 for the delivery of matrix-tethered therapeutic domains to the gut
Pichet Praveschotinunt,Pichet Praveschotinunt,Anna M. Duraj-Thatte,Anna M. Duraj-Thatte,Ilia Gelfat,Ilia Gelfat,Franziska Bahl,Franziska Bahl,David B. Chou,David B. Chou,Neel Joshi,Neel Joshi +11 more
TL;DR: This work genetically engineer E. coli Nissle 1917 to create a fibrous matrix that has a protective effect in DSS-induced colitis mice, and lays a foundation for the development of a platform in which the in situ production of therapeutic protein matrices from beneficial bacteria can be exploited.
Journal ArticleDOI
Genetically Programmable Self-Regenerating Bacterial Hydrogels
Anna M. Duraj-Thatte,Anna M. Duraj-Thatte,Noémie-Manuelle Dorval Courchesne,Noémie-Manuelle Dorval Courchesne,Pichet Praveschotinunt,Pichet Praveschotinunt,Jarod Rutledge,Jarod Rutledge,Yuhan Lee,Yuhan Lee,Yuhan Lee,Jeffrey M. Karp,Neel Joshi,Neel Joshi +13 more
TL;DR: An ELM that uses Escherichia coli as its cellular chassis and engineered curli nanofibers as its extracellular matrix component is demonstrated, laying a foundation for the application of ELMs with therapeutic functions and extended residence times in the gut.
Journal ArticleDOI
Modulating bacterial and gut mucosal interactions with engineered biofilm matrix proteins.
Anna M. Duraj-Thatte,Anna M. Duraj-Thatte,Pichet Praveschotinunt,Pichet Praveschotinunt,Trevor R. Nash,Trevor R. Nash,Frederick R. Ward,Neel Joshi,Neel Joshi +8 more
TL;DR: The use of engineered curli fibers are demonstrated to rationally program interactions between bacteria and components of the mucosal epithelium to demonstrate the potential utility of curli fiber as a scaffold for the display of bioactive domains and an untapped approach to r logically modulating host-microbe interactions using bacterial matrix proteins.
Journal ArticleDOI
Plasmid Vectors for in Vivo Selection-Free Use with the Probiotic E. coli Nissle 1917.
Anton Kan,Ilia Gelfat,Ilia Gelfat,Sivaram Emani,Sivaram Emani,Pichet Praveschotinunt,Pichet Praveschotinunt,Neel Joshi,Neel Joshi,Neel Joshi +9 more
TL;DR: This work systematically engineered both pMUT plasmids to contain selection markers, fluorescent markers, temperature sensitive expression, and curli secretion systems to export a customizable functional material into the extracellular space, presenting a platform for the rapid development of therapeutic EcN bacteria.