Showing papers by "Pierre-Yves Pontalier published in 2013"
TL;DR: The study spotted the light on the influence of the cell wall on evaluating the conversion factor and protein extractability, and proved that there is not a universal conversion factor that can be recommended.
Abstract: Additional evidence about the influence of the cell wall physical and chemical characteristics on protein extractability was determined by calculating the conversion factors of five different microalgae known to have different cell wall composition, and their protein extracts. The conversion factors obtained for crude rigid cell walled Chlorella vulgaris, Nannochloropsis oculata and Haematococcus pluvialis were 6.35, 6.28 and 6.25, respectively, but for their protein extracts the values were lower with 5.96, 5.86 and 5.63. On the other hand, conversion factor obtained for fragile cell walled microalgae Porphyridium cruentum and Athrospira platensis was 6.35 for the former and 6.27 for the latter, with no significant difference for their protein extract with 6.34 for the former and 6.21 for the latter. In addition, the highest hydro-soluble protein percentage recovered from total protein was for P. cruentum 80.3 % and A. platensis 69.5 % but lower for C. vulgaris with 43.3 %, N. oculata with 33.3 % and H. pluvialis with 27.5 %. The study spotted the light on the influence of the cell wall on evaluating the conversion factor and protein extractability. In addition, it showed the necessity of finding the conversion factor every time accurate protein quantification is required, and proved that there is not a universal conversion factor that can be recommended.
197 citations
TL;DR: In this article, the authors developed a new approach to green plant fractionation using twin-screw extrusion, leading to the production of a green filtrate rich in proteins and a solid fibrous residue, rich in cellulose.
37 citations
TL;DR: The amino acid profile of the red microalga Porphyridium cruentum and its protein extract and its amino acid sequence compares favourably with the standard protein/amino acid requirements proposed by Food and Agricultural Organisation and World Health Organisation.
Abstract: The amino acid profile of the red microalga Porphyridium cruentum and its protein extract have been determined in order to assess the nutritional quality of this biomass for human consumption. Total protein determined by elemental analysis represented 56 % of its dry weight. Hydro-soluble proteins extracted at pH 12 and 40 °C were analysed by the Lowry method giving 47 %, which represented 84 % of total protein per dry weight. The amino acid sequence of the biomass and the protein extract was composed of a set of essential (39 % for the former and 37 % for the latter) and non-essential amino acids (61 % for the former and 63 % for the latter) that compares favourably with the standard protein/amino acid requirements proposed by Food and Agricultural Organisation and World Health Organisation.
28 citations
TL;DR: In this paper, the influence of the screw profile on protein recovery in the liquid extract and on plant dehydration was characterised, and a significant difference was determined, at a confidence level of 95%, between the three profiles on alfalfa dehydration and protein extraction in the raw filtrate.
Abstract: Alfalfa was studied to define a new approach for green plant fractionation using twin-screw extrusion, leading to the production of a green filtrate, rich in proteins and a solid fibrous residue, rich in cellulose. The influence of the screw profile on protein recovery in the liquid extract and on plant dehydration was characterised. Three screw profiles were tested, at different liquid/solid ratios and plant inlet flow rates. A significant difference was determined, at a confidence level of 95%, between the influence of the three profiles on alfalfa dehydration and protein extraction in the raw filtrate. When mechanical action on the plant is stronger, both protein recovery and solid dehydration are better with more than half of the initial proteins (50.8%) recovered in the liquid extract in the best screw configuration (profile 3). However, extrusion is not efficient for the plant dehydration but the interest of adding a second filtration zone was demonstrated; after the first pressing and liquid expression in a first stage, water is added to the residual matter, which leads to a better protein extraction in the second filtration stage.
27 citations
01 Jan 2013
TL;DR: In this paper, a twin-screw extruder is used to extract oil from sunflower whole plant, which is then used as co-emulsifier for creams production in cosmetic industry.
Abstract: Biorefinery of sunflower whole plant is conducted according to an aqueous process using a twin-screw extruder. Aqueous extraction of oil is looked upon as an environmentally cleaner alternative technology to solvent extraction. Twin-screw extruder carries out three unit operations continuously: conditioning and grinding of whole plant, liquid/solid extraction and liquid/solid separation. Extraction efficiency depends on screw speed, and input flow rates of whole plant and water. In best conditions, oil yield is 57%, and residual oil content in cake meal is 14%. These conditions lead to the co-extraction of proteins, pectins and hemicelluloses. Oil is extracted in the form of two oil-in-water emulsions stabilized by phospholipids and proteins at interface. They could be used as co-emulsifiers for creams production in cosmetic industry. An aqueous extract containing part of the water-soluble constituents from whole plant, mainly proteins and pectins, is also generated. It can be recycled to the process. As a mixture of fibers and proteins, the cake meal can be moulded by thermo-pressing. Denser fiberboards have promising mechanical properties in bending. They could be used in furniture industry. Fiberboards with the lowest densities are more fragile but they could be used for their heat insulation properties in building industry.