P
Prakash Parajuli
Researcher at Sun Moon University
Publications - 52
Citations - 946
Prakash Parajuli is an academic researcher from Sun Moon University. The author has contributed to research in topics: Chemistry & Engineering. The author has an hindex of 13, co-authored 44 publications receiving 741 citations.
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Microbial production of natural and non-natural flavonoids: Pathway engineering, directed evolution and systems/synthetic biology.
TL;DR: All the manipulations/engineering accomplished on the microorganisms since 2000 are described in detail along with the biosynthetic pathway enzymes, their sources, structures of the compounds, and yield of each product.
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Assessing acceptor substrate promiscuity of YjiC-mediated glycosylation toward flavonoids
Ramesh Prasad Pandey,Rit Bahadur Gurung,Prakash Parajuli,Niranjan Koirala,Le Thi Tuoi,Jae Kyung Sohng +5 more
TL;DR: The acceptor substrate promiscuity of YjiC, a UDP-glycosyltransferase from Bacillus licheniformis, was explored with seven different classes of flavonoids and the enzymatic bioconversion was significantly higher with the production of multiple glucosylated derivatives.
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Methylation and subsequent glycosylation of 7,8-dihydroxyflavone.
TL;DR: Biotransformation of 7,8-DHF in a grown-induced culture of E. coli BL21 (DE3) harboring the recombinant pET-28a-SpOMT2884 stoichiometrically converted 7-hydroxy-8-methoxyflavone, suggesting that methylation enhances the stability of substrate and glycosylation has proved to increase the water solubility.
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Enzymatic Biosynthesis of Novel Resveratrol Glucoside and Glycoside Derivatives
Ramesh Prasad Pandey,Prakash Parajuli,Ju Yong Shin,Jisun Lee,Seul Ki Lee,Young-Soo Hong,Yong Il Park,Joong Su Kim,Jae Kyung Sohng +8 more
TL;DR: In this article, a UDP glucosyltransferase from Bacillus licheniformis was overexpressed, purified, and incubated with nucleotide diphosphate (NDP) d- and l-sugars to produce glucose, galactose, 2-deoxyglucose, viosamine, rhamnose, and fucose sugar-conjugated resveratrol glycosides.
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Glucosylation of isoflavonoids in engineered Escherichia coli.
Ramesh Prasad Pandey,Prakash Parajuli,Niranjan Koirala,Joo-Ho Lee,Yong Il Park,Jae Kyung Sohng +5 more
TL;DR: The in vivo fermentation of the isoflavonoids by applying engineered E. coli BL21(DE3)/ΔpgiΔzwfΔushA overexpressing phosphoglucomutase (pgm) and glucose 1-phosphate uridyltransferase (galU), along with YjiC, found more than 60% average conversion of 200 μM of supplemented is oflavonoid glucosides, without any additional UDP-α