Journal ArticleDOI
Methylation and subsequent glycosylation of 7,8-dihydroxyflavone.
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TLDR
Biotransformation of 7,8-DHF in a grown-induced culture of E. coli BL21 (DE3) harboring the recombinant pET-28a-SpOMT2884 stoichiometrically converted 7-hydroxy-8-methoxyflavone, suggesting that methylation enhances the stability of substrate and glycosylation has proved to increase the water solubility.About:
This article is published in Journal of Biotechnology.The article was published on 2014-08-20. It has received 55 citations till now. The article focuses on the topics: Glycosylation & Streptomyces peucetius.read more
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Microbial production of natural and non-natural flavonoids: Pathway engineering, directed evolution and systems/synthetic biology.
TL;DR: All the manipulations/engineering accomplished on the microorganisms since 2000 are described in detail along with the biosynthetic pathway enzymes, their sources, structures of the compounds, and yield of each product.
Journal ArticleDOI
Methylation of flavonoids: Chemical structures, bioactivities, progress and perspectives for biotechnological production.
TL;DR: Analysis of flavonoid methoxides including O- and C-methoxide derivatives in aspect of structure, bioactivities and description of almost all up-to-date O-and C-methyltransferases' enzymatic characteristics are concentrated on.
Journal ArticleDOI
Recent developments in the enzymatic O-glycosylation of flavonoids
TL;DR: The glycosylation of bioactive compounds, such as flavonoids, is of particular relevance, as it modulates many of their pharmacokinetic parameters, and the development of appropriate high-throughput screening systems appears to be the major bottleneck for this powerful technology.
Journal ArticleDOI
Production, Characterization, and Industrial Application of Pectinase Enzyme Isolated from Fungal Strains
K C Sudeep,Jitendra Upadhyaya,Dev Raj Joshi,Binod Lekhak,Dhiraj Kumar Chaudhary,Bhoj Raj Pant,Tirtha Raj Bajgai,Rajiv Dhital,Santosh Khanal,Niranjan Koirala,Vijaya Raghavan +10 more
TL;DR: In this article, Aspergillus spp. Gm was identified as a potent strain for pectinase production in commercial scale, and it was shown that the enzyme was produced by submerged state fermentation and assayed using the dinitro salicylic acid (DNS) method.
Journal ArticleDOI
Treatment with the flavonoid 7,8-Dihydroxyflavone: a promising strategy for a constellation of body and brain disorders.
Marco Emili,Sandra Guidi,Beatrice Uguagliati,Andrea Giacomini,Renata Bartesaghi,Fiorenza Stagni +5 more
TL;DR: The time is now ripe to move from the bench to the bedside, in order to establish whether supplementation with 7,8-DHF may serve as therapy or, at least, as adjuvant for the treatment of pathologies affecting brain and body functioning.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
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The neighbor-joining method: a new method for reconstructing phylogenetic trees.
Naruya Saitou,Masatoshi Nei +1 more
TL;DR: The neighbor-joining method and Sattath and Tversky's method are shown to be generally better than the other methods for reconstructing phylogenetic trees from evolutionary distance data.
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The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.
TL;DR: ClUSTAL X is a new windows interface for the widely-used progressive multiple sequence alignment program CLUSTAL W, providing an integrated system for performing multiple sequence and profile alignments and analysing the results.
Journal ArticleDOI
MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0
TL;DR: An advanced version of the Molecular Evolutionary Genetics Analysis software, which currently contains facilities for building sequence alignments, inferring phylogenetic histories, and conducting molecular evolutionary analysis, is released, which enables the inference of timetrees, as it implements the RelTime method for estimating divergence times for all branching points in a phylogeny.
Brief Communication MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0
TL;DR: The Molecular Evolutionary Genetics Analysis (MEGA) software as discussed by the authors provides facilities for building sequence alignments, inferring phylogenetic histories, and conducting molecular evolutionary analysis, including the inference of timetrees.