P
Purvish P. Patel
Publications - 21
Citations - 2822
Purvish P. Patel is an academic researcher. The author has contributed to research in topics: Immunoassay & Chemistry. The author has an hindex of 12, co-authored 19 publications receiving 2206 citations.
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Journal ArticleDOI
Single-Molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations
David M. Rissin,Cheuk W. Kan,Todd G. Campbell,Stuart C. Howes,David Fournier,Linan Song,Tomasz Piech,Purvish P. Patel,Lei Chang,Andrew J. Rivnak,Evan P. Ferrell,Jeffrey Randall,Gail K. Provuncher,David R. Walt,David C. Duffy +14 more
TL;DR: The authors' single-molecule enzyme-linked immunosorbent assay (digital ELISA) approach detected as few as ∼10–20 enzyme-labeled complexes in 100 μl of sample and routinely allowed detection of clinically relevant proteins in serum at concentrations much lower than conventional ELISA.
Journal ArticleDOI
The Simoa HD-1 Analyzer: A Novel Fully Automated Digital Immunoassay Analyzer with Single-Molecule Sensitivity and Multiplexing.
David H. Wilson,David M. Rissin,Cheuk W. Kan,David Fournier,Tomasz Piech,Todd G. Campbell,Raymond E. Meyer,Matthew W. Fishburn,Carlos Cabrera,Purvish P. Patel,Erica Frew,Yao Chen,Lei Chang,Evan P. Ferrell,Volker von Einem,William McGuigan,Marcus Reinhardt,Heiko Sayer,Claus Vielsack,David C. Duffy +19 more
TL;DR: A new laboratory instrument is described that provides full automation of single-molecule array (Simoa) technology for digital immunoassays that was illustrated in two clinical areas: traumatic brain injury and early detection of infectious disease.
Journal ArticleDOI
Simultaneous detection of single molecules and singulated ensembles of molecules enables immunoassays with broad dynamic range.
David M. Rissin,David Fournier,Tomasz Piech,Cheuk W. Kan,Todd G. Campbell,Linan Song,Lei Chang,Andrew J. Rivnak,Purvish P. Patel,Gail K. Provuncher,Evan P. Ferrell,Stuart C. Howes,Brian A. Pink,Kaitlin A. Minnehan,David H. Wilson,David C. Duffy +15 more
TL;DR: The combined digital and analog PSA assay provided linear response over approximately four logs of concentration, and the dynamic range of ELISA from picomolar levels down to subfemtomolar levels in a single measurement was extended.
Journal ArticleDOI
Hypoxia Due to Cardiac Arrest Induces a Time-Dependent Increase in Serum Amyloid β Levels in Humans
Henrik Zetterberg,Erik Mörtberg,Linan Song,Lei Chang,Gail K. Provuncher,Purvish P. Patel,Evan P. Ferrell,David Fournier,Cheuk W. Kan,Todd G. Campbell,Ray E. Meyer,Andrew J. Rivnak,Brian A. Pink,Kaitlin A. Minnehan,Tomasz Piech,David M. Rissin,David C. Duffy,Sten Rubertsson,David H. Wilson,Kaj Blennow +19 more
TL;DR: These data provide the first direct evidence in living humans that ischemia acutely increases Aβ levels in blood and point to the possibility that hypoxia may play a role in the amyloidogenic process of AD.
Journal ArticleDOI
Single molecule enzyme-linked immunosorbent assays: theoretical considerations.
Lei Chang,David M. Rissin,David Fournier,Tomasz Piech,Purvish P. Patel,David H. Wilson,David C. Duffy +6 more
TL;DR: The theoretical basis for the design of this assay derived from simple equations based on bimolecular interactions is provided, which makes it possible to directly compare the number of molecules detected experimentally to those predicted by theory.