Journal of Laboratory Automation 

About: Journal of Laboratory Automation is an academic journal. The journal publishes majorly in the area(s): Laboratory automation & Automation. It has an ISSN identifier of 2472-6303. Over the lifetime, 1025 publications have been published receiving 14337 citations.

TEER Measurement Techniques for In Vitro Barrier Model Systems

Abstract: Transepithelial/transendothelial electrical resistance (TEER) is a widely accepted quantitative technique to measure the integrity of tight junction dynamics in cell culture models of endothelial and epithelial monolayers. TEER values are strong indicators of the integrity of the cellular barriers before they are evaluated for transport of drugs or chemicals. TEER measurements can be performed in real time without cell damage and generally are based on measuring ohmic resistance or measuring impedance across a wide spectrum of frequencies. The measurements for various cell types have been reported with commercially available measurement systems and also with custom-built microfluidic implementations. Some of the barrier models that have been widely characterized using TEER include the blood–brain barrier (BBB), gastrointestinal (GI) tract, and pulmonary models. Variations in these values can arise due to factors such as temperature, medium formulation, and passage number of cells. The aim of this article ...

National Instruments LabVIEW: A Programming Environment for Laboratory Automation and Measurement

Abstract: National Instruments LabVIEW is a graphical programming language that has its roots in automation control and data acquisition. Its graphical representation, similar to a process flow diagram, was created to provide an intuitive programming environment for scientists and engineers. The language has matured over the last 20 years to become a general purpose programming environment. LabVIEW has several key features which make it a good choice in an automation environment. These include simple network communication, turnkey implementation of common communication protocols (RS232, GPIB, etc.), powerful toolsets for process control and data fitting, fast and easy user interface construction, and an efficient code execution environment. We discuss the merits of the language and provide an example application suite written in-house which is used in integrating and controlling automation platforms.

The Simoa HD-1 Analyzer: A Novel Fully Automated Digital Immunoassay Analyzer with Single-Molecule Sensitivity and Multiplexing.

Abstract: Disease detection at the molecular level is driving the emerging revolution of early diagnosis and treatment. A challenge facing the field is that protein biomarkers for early diagnosis can be present in very low abundance. The lower limit of detection with conventional immunoassay technology is the upper femtomolar range (10−13 M). Digital immunoassay technology has improved detection sensitivity three logs, to the attomolar range (10−16 M). This capability has the potential to open new advances in diagnostics and therapeutics, but such technologies have been relegated to manual procedures that are not well suited for efficient routine use. We describe a new laboratory instrument that provides full automation of single-molecule array (Simoa) technology for digital immunoassays. The instrument is capable of single-molecule sensitivity and multiplexing with short turnaround times and a throughput of 66 samples/h. Singleplex and multiplexed digital immunoassays were developed for 16 proteins of interest in ...

A mechanical biomarker of cell state in medicine.

Abstract: The mechanical properties of cells have been shown to be useful markers of cell state by the biophysics community. Here, I highlight clinical and research problems that this label-free, potentially inexpensive cellular biomarker can address and discuss technical challenges to realize automated instruments to achieve robust and high-throughput mechanical measurements. Important features found in traditional fluorescence-based flow cytometry that can enable cytometry based on mechanical properties (i.e., deformability cytometry) are emphasized, especially the need for throughput, simple operation, multidimensional data visualization, and internal controls. Next-generation approaches to automate deformability measurements of cells are surveyed, and future directions are outlined that promise to bring low-cost mechanical measurements to medicine and biological research.

Development and Applications of Portable Biosensors

Abstract: The significance of microfluidics-based and microelectromechanical systems-based biosensors has been widely acknowledged, and many reviews have explored their potential applications in clinical diagnostics, personalized medicine, global health, drug discovery, food safety, and forensics. Because health care costs are increasing, there is an increasing need to remotely monitor the health condition of patients by point-of-care-testing. The demand for biosensors for detection of biological warfare agents has increased, and research is focused on ways of producing small portable devices that would allow fast, accurate, and on-site detection. In the past decade, the demand for rapid and accurate on-site detection of plant disease diagnosis has increased due to emerging pathogens with resistance to pesticides, increased human mobility, and regulations limiting the application of toxic chemicals to prevent spread of diseases. The portability of biosensors for on-site diagnosis is limited due to various issues, including sample preparation techniques, fluid-handling techniques, the limited lifetime of biological reagents, device packaging, integrating electronics for data collection/analysis, and the requirement of external accessories and power. Many microfluidic, electronic, and biological design strategies, such as handling liquids in biosensors without pumps/valves, the application of droplet-based microfluidics, paper-based microfluidic devices, and wireless networking capabilities for data transmission, are being explored.