Showing papers in "Journal of Immunological Methods in 2012"

TL;DR: This study validated CD80 as the most robust phenotypic marker for human MΦ(IFN-γ), whereas CD200R was upregulated and CD14 was specifically downregulated on M Φ(IL-4) and CD163 and CD16 were found to be specific markers for M Δ�( IL-10).

TL;DR: A non-radiolabeled homogeneous mobility shift assay (HMSA) to measure the antibodies-to-infliximab (ATI) and IFX levels in serum samples that may be applied to other protein-based drugs to accurately detect serum drug and anti-drug antibody levels.

TL;DR: A multiplex PCR method to sequence tens of millions of T cell receptors (TCRs) from a single sample in a few days is developed, able to detect even the rarest clones.

TL;DR: The key finding is that use of citrate or EDTA anticoagulants decreases or eliminates microvesicles from plasma by inducing adhesion of the microvesicle to platelets or other formed elements.

TL;DR: The data generated in this array provides a profile of the humoral immune response with multi-dimensional metrics regarding properties of both variable and constant IgG domains, and can be used to predict functions such as antibody effector activity.

TL;DR: If samples have to be cryopreserved, monitoring of CD11b+ and CD14+ MDSC subsets provides the most reliable results.

TL;DR: While both CTV and CPD can label cells to a high fluorescence intensity, which is long-lived and has low variability and low toxicity and makes them ideal for long-term tracking of non-dividing lymphocytes in vivo, CTV offers possibly the best available alternative to CFSE in the analysis of cell divisions.

TL;DR: The theoretical basis for the design of this assay derived from simple equations based on bimolecular interactions is provided, which makes it possible to directly compare the number of molecules detected experimentally to those predicted by theory.

TL;DR: While studying the interactions of the vaccinia virus complement control protein (VCP) with complement, non-specific binding of VCP to BSA is found and the need to perform critical controls to ensure that ELISA reactants do not inappropriately bind to the blocking agent is highlighted.

TL;DR: Comparing the detection limit between the four most used labels, carbon black was used in a detection system for Dengue fever and the colorimetric detection limit was ten times lower than the 575 ng/mL observed for standard gold nanoparticles; which makes it sensitive enough to diagnose a patient on the first days of infection.

TL;DR: Unlabelled protein immunoprecipitation is a specific analytical approach, appropriate for the identification of the recently described anti-SAE autoantibody, and the role of anti- SAE antibody as marker of dermatomyositis is confirmed.

TL;DR: A fast HPLC profiling method was developed for the separation and quantitation of IgG glycans (neutral, G0, G1, and G2, mono- and di-sialylated) using simple procedures and should prove useful for monitoring glycan changes in clinical settings.

TL;DR: It is demonstrated that this aspecific antibody binding can be inhibited by adding F(ab')2 of intravenous immunoglobulin (IVIG), which consists of pooled polyclonal IgG derived from plasma, and anti-infliximab antibodies can be measured specifically without interference by anti-hinge antibodies.

TL;DR: A reproducible nasal allergen challenge technique is developed and Tryptase in local nasal secretions is a potential biomarker of the early phase response; ECP and the Th2 cytokines IL-5, -13 and -4 markers of late phase allergic responses are studied.

TL;DR: A novel assay to study leukocyte margination and trafficking following pulmonary inflammation is developed and shows that marginated cells comprise a large fraction of lung leukocytes that increases shortly after lung injury.

TL;DR: This study provides the proof-of-concept that histamine and its release can be studied by multicolor flow cytometry on a single cell level (HistaFlow).

TL;DR: Cancer vaccines are capable of generating an active tumor-specific immune response and serve as an ideal treatment due to their specificity for tumor cells and long lasting immunological memory that may safeguard against recurrences.

TL;DR: This Standardized Proficiency Testing Program is the first available for the evaluation and documentation of assay equivalency for laboratories performing HIV-1 neutralizing antibody assays and may provide guidance for the development of future proficiency testing programs for other assay platforms.

TL;DR: The design and testing of a new set of rhesus-specific primers that enable efficient and specific amplification of heavy, kappa and lambda V(D)J genes from single sorted B cells are described.

TL;DR: The use of a single lateral flow device that can detect and distinguish between BoNT/A and B, two of the four serotypes that are known to intoxicate humans and together represent >80% of naturally occurring illness, is described.

TL;DR: It is concluded that the RFADCC assay primarily reflects Ab-mediated monocyte function, and further studies on the immunological importance of HIV-specific monocyte-mediated ADCC are warranted.

TL;DR: A new hybrid method to simulate tumor growth and immune system, by applying Cellular Automata Lattice Boltzmann (CA-LB) approach, which is replaced with a statistic and stochastic model based on the numerical discretization of the BoltZmann equation.

TL;DR: The recovery and viability of TEC, notably the rare cortical subsets, were significantly enhanced with Liberase products containing medium to high levels of thermolysin, and improved stromal dissociation led to numerically increased TEC yield and total TEC RNA isolated from pooled digests of adult thymus.

TL;DR: A novel methodology for the identification and quantitation of CEC and EMP based on multicolor flow cytometry is presented, which may be useful for monitoring endothelial dysfunction in clinical settings.

TL;DR: This ELISA offers a convenient and reliable method for studying CL-11 levels in relation to a variety of human diseases and syndromes and shows excellent quantitative characteristics such as reproducibility, dilution linearity and recovery.

TL;DR: It is suggested that the majority of ELISAs work reliably with saliva, even if the assay was not specifically designed for this biological fluid, although a few cases where recovery or intra-and inter-assay variations were unexpectedly high are reported.

TL;DR: The Anterior Gradient (AGR) genes AGR2 and AGR3 are part of the PDI family and harbor core thioredoxin folds (CxxS motifs) that have the potential to regulate protein folding and maturation as discussed by the authors.

TL;DR: Using tumor growth data from a large number of mice challenged with live tumor cells, the use of area under the curve (AUC) is described, demonstrating the utility of this tool over the conventional methods for these purposes.

TL;DR: The reported expression of Foxp3 in macrophages is likely an artifact and that a stringent multimodality approach is critical to demonstrate candidate gene expression in any cell type.

TL;DR: The validation and long term performance characteristics of the assay during its six year application in the AVRP are reported, confirming its suitability for long term studies of PA protein-based anthrax vaccines.