Rapid improvements in sequencing and array-based platforms are resulting in a flood of diverse genome-wide data, including data from exome and whole-genome sequencing, epigenetic surveys, expression profiling of coding and noncoding RNAs, single nucleotide polymorphism (SNP) and copy number profiling, and functional assays. Analysis of these large, diverse data sets holds the promise of a more comprehensive understanding of the genome and its relation to human disease. Experienced and knowledgeable human review is an essential component of this process, complementing computational approaches. This calls for efficient and intuitive visualization tools able to scale to very large data sets and to flexibly integrate multiple data types, including clinical data. However, the sheer volume and scope of data pose a significant challenge to the development of such tools.

Integrative Genomics Viewer

Universal sample preparation method for proteome analysis

Opportunities and challenges in biological lignin valorization

Dispersed repetitive DNA sequences from yeast (Saccharomyces cerevisiae) nuclear DNA have been isolated as molecular hybrids in lambdagt. Related S. cerevisiae strains show marked alterations in the size of the restriction fragments containing these repetitive DNAs. "Ty1" is one such family of repeated sequences in yeast and consists of a 5.6 kilobase (kb) sequence including a noninverted 0.25 kb sequence of another repetitious family, "delta", on each end. There are about 35 copies of Ty1 and at least 100 copies of delta (not always associated with Ty1) in the haploid genome. A few Ty1 elements are tandem and/or circular, but most are disperse and show (along with delta) some sequence divergence between repeat units. Sequence alterations involving Ty1 elements have been found during the continual propagation of a single yeast clone over the course of a month. One region with a large number of delta sequences (SUP4) also shows a high frequency of sequence alterations when different strains are compared. One of the differences between two such strains involves the presence or absence of a Ty1 element. The novel joint is at one inverted pair of delta sequences.

Evidence for transposition of dispersed repetitive DNA families in yeast [Saccharomyces cerevisiae]

Augmented digestion of lignocellulose by steam explosion, acid and alkaline pretreatment methods: a review.

Relationship among content type of Smartphone Use, Technostress, and Sleep Difficulty: a study of University students in China

p-coumaric acid is one of the aromatic compounds that are widely used in food, cosmetics and medicine due to its properties of antibacterium, antioxidation and cardiovascular disease prevention. Tyrosine ammonia-lyase (TAL) catalyzes the deamination of tyrosine to p-coumaric acid. However, the lack of highly active and specific tyrosine ammonia lyase limits cost-effective microbial production of p-coumaric acid. In order to improve biosynthesis efficiency of p-coumaric acid, two tyrosine ammonia-lyases, namely Fc-TAL2 derived from Flavobacterium columnare and Fs-TAL derived from Flavobacterium suncheonense, were selected and characterized. The optimum temperature (55 ℃) and pH (9.5) for Fs-TAL and Fc-TAL2 are the same. Under optimal conditions, the specific enzyme activity of Fs-TAL and Fc-TAL2 were 82.47 U/mg and 13.27 U/mg, respectively. Structural simulation and alignment analysis showed that the orientation of the phenolic hydroxyl group of the conserved Y50 residue on the inner lid loop and its distance to the substrate were the main reasons accounting for the higher activity of Fs-TAL than that of Fc-TAL2. The higher activity and specificity of Fs-TAL were further confirmed via whole-cell catalysis using recombinant Escherichia coli, which could convert 10 g/L tyrosine into 6.2 g/L p-coumaric acid with a yield of 67.9%. This study provides alternative tyrosine ammonia-lyases and may facilitate the microbial production of p-coumaric acid and its derivatives.

[Characterization of highly active tyrosine ammonia lyase and its application in biosynthesis of p-coumaric acid].

Mobile English learning has multiple advantages and brings enormous benefits to EFL learners. However, not everyone adopts it, and the determinants of learners’ adoption intention have not yet been investigated fully. This study aimed to better understand learners’ adoption by employing the theory of planned behaviour (TPB) in conjunction with the influences of technostress and compatibility. Based on existing literature, a research model was proposed and verified with a sample of 409 undergraduates from a Chinese university. The results indicated that in the context of mobile English learning: (a) Individuals with higher adoption intention are more likely to engage in mobile English learning with higher frequency (b = 0.473, P < 0.001) and longer duration (b = 0.330, P < 0.001); (b) Individuals’ attitude toward mobile English learning (b = 0.171, P < 0.05), perceived behavioural control (b = 0.221, P < 0.001), subjective norms (b = 0.237, P < 0.05), and compatibility (b = 0.443, P < 0.001) are significantly positively associated with their adoption intention; (c) Compatibility is the strongest predictor of adoption intention (b = 0.443, P < 0.001) and negatively moderates the effect of subjective norms on adoption intention (b = –0.103, P < 0.005); (d) The influence of technostress on the adoption intention of mobile English learning is not significant (b = –0.041, P > 0.05). Practical implications related to mobile English learning were discussed.

/pdf/examining-the-moderating-role-of-technostress-and-15m4wamv.pdf

Examining the moderating role of technostress and compatibility in EFL Learners’ mobile learning adoption: A perspective from the theory of planned behaviour

Microbial protein manufacturing: The developing trend and challenge

As a steroidal drug intermediate, 16,17-epoxysterol plays an important role in pharmaceutical steroid synthesis. Current methods for epoxysterol determination normally require expensive equipment, ...

Qinhong Wang

Papers

Relationship among content type of Smartphone Use, Technostress, and Sleep Difficulty: a study of University students in China

[Characterization of highly active tyrosine ammonia lyase and its application in biosynthesis of p-coumaric acid].

Examining the moderating role of technostress and compatibility in EFL Learners’ mobile learning adoption: A perspective from the theory of planned behaviour

Microbial protein manufacturing: The developing trend and challenge

A Simple Colorimetric Method for Detection of 16, 17-Epoxyprogesterone