Nutrient requirements of dairy cattle , Nutrient requirements of dairy cattle , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی

Nutrient requirements of dairy cattle

Breast cancer is a complex and heterogeneous disease. Gene expression profiling has contributed significantly to our understanding of this heterogeneity at a molecular level, refining taxonomy based on simple measures such as histological type, tumour grade, lymph node status and the presence of predictive markers like oestrogen receptor and human epidermal growth factor receptor 2 (HER2) to a more sophisticated classification comprising luminal A, luminal B, basal-like, HER2-positive and normal subgroups. In the laboratory, breast cancer is often modelled using established cell lines. In the present review we discuss some of the issues surrounding the use of breast cancer cell lines as experimental models, in light of these revised clinical classifications, and put forward suggestions for improving their use in translational breast cancer research.

/pdf/choosing-the-right-cell-line-for-breast-cancer-research-2vywvl4eet.pdf

Choosing the right cell line for breast cancer research

Undesirable side effects of selection for high production efficiency in farm animals: a review

A number of genetic mutations have been identified in human breast cancers, yet the specific combinations of mutations required in concert to form breast carcinoma cells remain unknown. One approach to identifying the genetic and biochemical alterations required for this process involves the transformation of primary human mammary epithelial cells (HMECs) to carcinoma cells through the introduction of specific genes. Here we show that introduction of three genes encoding the SV40 large-T antigen, the telomerase catalytic subunit, and an H-Ras oncoprotein into primary HMECs results in cells that form tumors when transplanted subcutaneously or into the mammary glands of immunocompromised mice. The tumorigenicity of these transformed cells was dependent on the level of ras oncogene expression. Interestingly, transformation of HMECs but not two other human cell types was associated with amplifications of the c-myc oncogene, which occurred during the in vitro growth of the cells. Tumors derived from the transformed HMECs were poorly differentiated carcinomas that infiltrated through adjacent tissue. When these cells were injected subcutaneously, tumors formed in only half of the injections and with an average latency of 7.5 weeks. Mixing the epithelial tumor cells with Matrigel or primary human mammary fibroblasts substantially increased the efficiency of tumor formation and decreased the latency of tumor formation, demonstrating a significant influence of the stromal microenvironment on tumorigenicity. Thus, these observations establish an experimental system for elucidating both the genetic and cell biological requirements for the development of breast cancer.

http://genesdev.cshlp.org/content/15/1/50.full.pdf

Human breast cancer cells generated by oncogenic transformation of primary mammary epithelial cells

Breast cancer is not a single disease, but is instead a collection of diseases that have distinct histopathological features, genetic and genomic variability, and diverse prognostic outcomes. Thus, no individual model would be expected to completely recapitulate this complex disease. Here, the models commonly used to investigate breast cancer including cell lines, xenografts and genetically engineered mice, are discussed to help address the question: what is the most powerful way to investigate this heterogeneous disease?

Modelling breast cancer: one size does not fit all

Mammary growth in Holstein cows during the dry period: quantification of nucleic acids and histology

Influence of Nutrition on Mammary Development in Pre- and Postpubertal Heifers

The growth and morphogenesis of mammary parenchyma varies substantially between species and is regulated by an array of systemic and local factors. Central to this regulation is the mammary fat pad, a matrix of adipose and connective tissue capable of mediating hormone action and synthesizing an array of growth regulatory molecules. In this article we highlight differences between the morphological development of the mammary parenchyma in rodents, humans, and ruminant dairy animals, placing emphasis on differences in the cellular composition and structure of the mammary fat pad. While a great deal remains to be understood about the ability of stroma to locally regulate mammary development, the significance of its contribution is becoming increasingly apparent. The actions of several steroid and peptide hormones appear to be mediated by an array of growth factors, proteases and extracellular matrix components synthesized by constituents of the mammary fat pad. Further, mammary adipose tissue represents a significant store of lipid which, by itself and through its derivatives, could influence the growth of mammary epithelium in diverse ways. This review describes the integral role of the mammary fat pad during mammogenesis, emphasizing the point that species differences must be addressed if local growth and morphogenic mechanisms within the mammary gland are to be resolved.

Regulation of Mammary Gland Growth and Morphogenesis by the Mammary Fat Pad: A Species Comparison

Lifetime milk production is maximized when dairy cows are pregnant during approximately 70% of each lactation. Between lactations, a nonlactating period is necessary for optimal milk production in the succeeding lactation. With cessation of milking, alveolar structure is largely maintained and little or no loss of cells occurs. However, increased apoptosis and cell proliferation, relative to that in lactating glands during the same stage of gestation, suggest that a nonlactating period serves to promote cell turnover prior to the next lactation. Even in the absence of pregnancy, mammary involution in dairy animals occurs at a slower rate than in rodents; alveolar structure is maintained for several weeks and lactation can be reinitiated after four weeks or more of involution. Although apoptosis appears to be initiated within a similar time frame to that in rodents, the maximum proportion of apoptotic epithelial cells appears to be lower than in rodents, and apoptosis may be accompanied by an initial increase in cell proliferation. The ability to manipulate apoptosis and cell proliferation during the nonlactating period and during lactation is expected to provide enormous benefits to the dairy industry.

R.M. Akers

Papers

Mammary growth in Holstein cows during the dry period: quantification of nucleic acids and histology

Influence of Nutrition on Mammary Development in Pre- and Postpubertal Heifers

Regulation of Mammary Gland Growth and Morphogenesis by the Mammary Fat Pad: A Species Comparison

Mammary involution in dairy animals.

Major Advances Associated with Hormone and Growth Factor Regulation of Mammary Growth and Lactation in Dairy Cows