R
Rachel Fleming
Publications - 24
Citations - 548
Rachel Fleming is an academic researcher. The author has contributed to research in topics: RNA & Body fluid. The author has an hindex of 9, co-authored 21 publications receiving 460 citations.
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Journal ArticleDOI
The development of a mRNA multiplex RT-PCR assay for the definitive identification of body fluids.
Rachel Fleming,SallyAnn Harbison +1 more
TL;DR: A multiplex PCR system using messenger RNA that can identify blood, saliva, semen and menstrual blood in individual stains or in mixtures of body fluids and targeted the co-isolation of RNA and DNA from the same sample.
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The use of bacteria for the identification of vaginal secretions
Rachel Fleming,SallyAnn Harbison +1 more
TL;DR: This work has incorporated the Lactobacilli markers into a mRNA multiplex system to produce an 11-plex assay that can identify circulatory blood, menstrual blood, saliva, semen (in the presence and absence of spermatozoa) and vaginal secretions.
Journal ArticleDOI
Forensic body fluid identification: state of the art
SallyAnn Harbison,Rachel Fleming +1 more
TL;DR: Current technologies for identifying body fluids are described including the use of RNA (mRNA and miRNA), epigenetics, spectroscopic techniques such as Raman spectroscopy and micro-spectrophotometry, biosensors, and immunochromatographic methods which are outlined alongside their strengths and weaknesses.
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A method for DNA and RNA co-extraction for use on forensic samples using the Promega DNA IQ™ system.
TL;DR: The successful modification of the Promega DNA IQ™ system is reported on to enable co-extraction of DNA and RNA from the same sample without compromising the potential DNA profile.
Journal ArticleDOI
Transcriptomic analysis of degraded forensic body fluids
TL;DR: This study demonstrates the practical application of MPS technology for the accurate analysis of degraded RNA from minimal samples and indicates the accurate detection of known body fluid markers in respective body fluids.