Radosław Ł. Gwarda

TL;DR: This work shows, that at low or high concentration of water in the mobile phase comprised methanol and some additives the chromatographic systems with the plates mentioned demonstrate normal- or reversed-phase liquid chromatography properties, respectively, for separation of peptides obtained.

TL;DR: It is shown, that the system type inversion results in significant change of selectivity of separation, which may be especially useful in 2D separation of complex samples of basic/amphoteric compounds such as peptides.

TL;DR: In this paper, the influence of the following variables such as adsorbent type, type and concentration of organic modifier in mobile phase and pH of the mobile phase buffer on retention of some synthetic peptides in reversed-phase high-performance thin-layer chromatography systems has been investigated.

TL;DR: In this article, the authors present some practical observations and conclusions related to another our paper [1] and concern staining of some synthetic oligopeptides with ninhydrin, which may help other authors to optimize a procedure of visualization of similar compounds.

TL;DR: It is proved, that due to different characteristic of adsorbents used in both techniques (RP HPLC and HPTLC), influence of ion-pairing reagents on retention of basic and/or amphoteric compounds also may be quite different.