R
Ralph W. F. Hardy
Researcher at DuPont
Publications - 22
Citations - 4619
Ralph W. F. Hardy is an academic researcher from DuPont. The author has contributed to research in topics: Nitrogenase & Photorespiration. The author has an hindex of 15, co-authored 22 publications receiving 4445 citations.
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Biological nitrogen fixation: A key to world protein
TL;DR: Electron micrographic analysis of the developmental morphology of the natural soybean symbiosis and C2H2-C2H4 analysis indicate that increasing N2-fixing activity from 12–35 days of age is accompanied by an increase in bacteroid number per cell, bacteroids number per vesicle and inclusions per bacteroid, which is consistent with a proposed mechanism.
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N(2) Fixation by Azospirillum brasilense and Its Incorporation into Host Setaria italica.
TL;DR: There was no evidence of direct bacterium-to-plant transport of fixed nitrogen, but rather a slow transfer suggesting the gradual death of bacteria and subsequent mineralization of their nitrogen, at least under growth-room conditions.
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Establishment of symbiosis between Rhizobium and plant cells in vitro.
TL;DR: A symbiotic nitrogen-fixing relationship has been established in culture between soybean cells and the bacterium Rhizobium.
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Isolation by crystallization of the MoFe protein of Azotobacter nitrogenase
TL;DR: The crystallized protein in combination with the Fe protein was essential for nitrogenase reactions including reduction of N2, C2H2, CN−, acrylonitrile and azide, and ATP-dependent H2 evolution; specific activity was 1488 nmoles H2 evolved/minute/mg MoFe protein.
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Nitrogenase from vanadium-grown Azotobacter: Isolation, characteristics, and mechanistic implications
TL;DR: In this paper, a nitrogenase, designated V-N2 ase, was extracted from Azotobacter vinelandii OP grown on N2 in medium supplemented with V in place of Mo.