Rasmus Hansen Kirkegaard

TL;DR: The discovery and cultivation of a completely nitrifying bacterium from the genus Nitrospira, a globally distributed group of nitrite oxidizers, and the genome of this chemolithoautotrophic organism encodes the pathways both for ammonia and nitrite oxidation.

TL;DR: In this article, the authors systematically explored the impact of a number of parameters on the observed microbial community: bead beating intensity, primer choice, extracellular DNA removal, and various PCR settings.

TL;DR: ampvis2, an R package designed for analysis of microbial community data in OTU-table format with focus on simplicity, reproducibility, and sample metadata integration, with a minimal set of intuitive commands, which includes flexible heatmaps and simplified ordination.

TL;DR: This work combines poly(A)-tailing and reverse transcription of SSU rRNA molecules with synthetic long-read sequencing to generate high-quality, full-length SSUrRNA sequences, without primer bias, at high throughput, and will enable expansion of the SSu rRNA reference databases by orders of magnitude, and contribute to a comprehensive census of the tree of life.

TL;DR: In this article, a high-throughput amplicon sequencing approach combining unique molecular identifiers (UMIs) with Oxford Nanopore Technologies (ONT) or Pacific Biosciences circular consensus sequencing, yielding high-accuracy single-molecule consensus sequences of large genomic regions.