Søren Michael Karst

TL;DR: In this article, the authors systematically explored the impact of a number of parameters on the observed microbial community: bead beating intensity, primer choice, extracellular DNA removal, and various PCR settings.

TL;DR: A lab-scale moving bed biofilm reactor with carrier material proved to sufficiently sustain enough biomass to allow anammox activity even at 10 °C, and microbial community analysis by 16S rRNA amplicon analysis revealed a relatively stable community composition over the entire experimental period.

TL;DR: The MiDAS taxonomy is a manual curation of the SILVA taxonomy that proposes a name for all genus-level taxa observed to be abundant by large-scale 16 S rRNA gene amplicon sequencing of full-scale activated sludge communities.

TL;DR: Assessment of the abundance and diversity of all proposed PAOs and GAOs in 18 Danish full-scale wastewater treatment plants with well-working biological nutrient removal over a period of 9 years using 16S rRNA gene amplicon sequencing found microbial community structure in all plants was relatively stable over time.

TL;DR: The aerobic growth of a pure culture of the ammonia-oxidizing thaumarchaeote Nitrososphaera gargensis is reported using cyanate as the sole source of energy and reductant; to the authors' knowledge, the first organism known to do so and suggest a previously unrecognized importance of cyanate in cycling of nitrogen compounds in the environment.