Reichel Samuel

TL;DR: A red-emitting dicyanomethylenedihydrofuran push-pull fluorophore is reengineered so that it is dark until photoactivated with a short burst of low-intensity violet light, which provides a new class of bright photoactivatable fluorophores needed for super-resolution imaging schemes that require active control of single molecule emission.

TL;DR: A red-emitting DCDHF fluorophore is reengineered so that it is dark until photoactivated with a short burst of low-intensity violet light, providing a new class of bright photoactivatable small-molecule fluorophores, which are needed for super-resolution imaging schemes that require active control of single-Molecule emission.

TL;DR: It is demonstrated that the azide-to-amine photoactivation process is generally applicable to a variety of push-pull chromophores, and the photophysical parameters including photoconversion quantum yield, photostability, and turn-on ratio are characterized.

TL;DR: Fluorophores derived from photoactivatable azide-pi-acceptor fluorogens or from a thermal reaction of an azide/pi acceptor fluorogen with an alkene or alkyne are disclosed in this paper.

TL;DR: A series of photoactivatable push-pull organic fluorophores, single molecules of which can be imaged in living cells, are designed that require active control of single-molecule emission.