Showing papers by "Rienk van Grondelle published in 1989"
TL;DR: Laser spectroscopy allows us to measure rates of energy transfer between pigments of the light harvesting system for the first time and these rates are correlated with models of theLight harvesting apparatus.
81 citations
TL;DR: Sedata of thylakoid membranes and isolated chlorophyll a/b -protein complexes from Prochlorothrix hollandica and from spinach were different and the molecular organization of the chlorophyller complexes of the prochlorophyte appeared strikingly different from the one in chloroplasts of higher plants and green algae.
16 citations
TL;DR: The structure of the bovine alpha-crystallin aggregate and its reaggregated isolated subunits has been studied by measurement of their absorption and linear dichroism spectra over the range 250-350 nm, and a considerable deviation from a spherical or tetrahedrally symmetric structure is inferred.
Abstract: The structure of the bovine α-crystallin aggregate and its reaggregated isolated subunits has been studied by measurement of their absorption and linear dichroism spectra over the range 250–350 nm. Also, changes in structure with respect to time have been monitored in this way. From the absorption spectra it appears that the aromatic residues in subunit aggregates are in the same chemical environment as those in native protein. The light scattering due to the size of the protein molecules increases when the proteins are kept in solution, this effect being much stronger for the subunits. The linear dichroism spectra point to strong structural ordering in α-crystallin, the absorption transition dipoles of the aromatic residues being preferentially aligned along the long axis of the molecules. Moreover, a considerable deviation from a spherical or tetrahedrally symmetric structure of α-crystallin is inferred. The subunit aggregates show less ordering and might be more spherical. When kept in solution, their structural order seems to be decreased. The linear dichroism spectra show absorption at 325 nm, which is not detectable in the normal absorption spectra.
12 citations