R
Robin M. Kaufhold
Researcher at United States Military Academy
Publications - 14
Citations - 584
Robin M. Kaufhold is an academic researcher from United States Military Academy. The author has contributed to research in topics: Chlamydia trachomatis & ELISPOT. The author has an hindex of 6, co-authored 11 publications receiving 552 citations.
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Journal ArticleDOI
Decline in Varicella-Zoster Virus (VZV)–Specific Cell-Mediated Immunity with Increasing Age and Boosting with a High-Dose VZV Vaccine
Myron J. Levin,Jeffrey G. Smith,Robin M. Kaufhold,Debra Barber,Anthony R. Hayward,Christina Y. Chan,Ivan S. F. Chan,David J. J. Li,William Wang,Paul M. Keller,Alan Shaw,Jeffrey L. Silber,Katia Schlienger,Ira Chalikonda,S. J. R. Vessey,Michael J. Caulfield +15 more
TL;DR: A live attenuated VZV vaccine is safe and immunogenic in an elderly population, and the vaccine-induced immunity may be monitored by the IFN-gamma ELISPOT assay, which had greater sensitivity and a wider dynamic range.
Journal ArticleDOI
Development and validation of a gamma interferon ELISPOT assay for quantitation of cellular immune responses to varicella-zoster virus.
TL;DR: A gamma interferon ELISPOT assay that provides a direct measure of the number of T cells secreting a cytokine following stimulation with antigen, which is extremely sensitive and specific for VZV-specific cell-mediated immunity.
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Establishing Acceptance Criteria for Cell-Mediated-Immunity Assays Using Frozen Peripheral Blood Mononuclear Cells Stored under Optimal and Suboptimal Conditions
Jeffrey G. Smith,Heather R. Joseph,Tina Green,Jodie A. Field,Melissa Wooters,Robin M. Kaufhold,Joseph M. Antonello,Michael J. Caulfield +7 more
TL;DR: For a given suboptimal storage event, the magnitude of the reduction varied between individuals and even among aliquots within an individual bleed, indicating the need for sample-specific acceptance criteria (AC).
Journal ArticleDOI
A real-time quantitative polymerase chain reaction assay for the detection of Chlamydia in the mouse genital tract model.
Melissa Wooters,Robin M. Kaufhold,Jodie A. Field,Lani Indrawati,Jon H. Heinrichs,Jeffrey G. Smith +5 more
TL;DR: A highly sensitive real-time quantitative polymerase chain reaction (RT-qPCR) assay for enumeration of Chlamydia from mouse genital tracts to increase assay sensitivity, remove subjectivity, and improve sample throughput is developed.
Journal ArticleDOI
A novel automated method for enumeration of Chlamydia trachomatis inclusion forming units
Su Wang,Lani Indrawati,Melissa Wooters,Ivette Caro-Aguilar,Jodie A. Field,Robin M. Kaufhold,Angela M. Payne,Michael J. Caulfield,Jeffrey G. Smith,Jon H. Heinrichs +9 more
TL;DR: In this article, an automated microplate ImmunoSpot reader was used to count C. trachomatis inclusion forming units (IFUs) in a 96-well plate and subsequent incubation.